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1) Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis (Nature Methods) Sunday July 20th 2014
George T Lyozin, Paul C Bressloff, Amit Kumar, Yasuhiro Kosaka, Bradley L Demarest, H Joseph Yost, Michael R Kuehn, Luca Brunelli

2) RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP) (Nature Methods) Sunday July 20th 2014
Nathan A Siegfried, Steven Busan, Greggory M Rice, Julie A E Nelson, Kevin M Weeks

3) Fast, accurate reconstruction of cell lineages from large-scale fluorescence microscopy data (Nature Methods) Sunday July 20th 2014
Fernando Amat, William Lemon, Daniel P Mossing, Katie McDole, Yinan Wan, Kristin Branson, Eugene W Myers, Philipp J Keller

4) High-resolution reconstruction of the beating zebrafish heart (Nature Methods) Sunday July 20th 2014
Michaela Mickoleit, Benjamin Schmid, Michael Weber, Florian O Fahrbach, Sonja Hombach, Sven Reischauer, Jan Huisken

5) Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity (Nature Methods) Sunday July 20th 2014
S, Heather J Lee, Christof Angermueller, Felix Krueger, Heba Saadeh, Julian Peat, Simon R Andrews, Oliver Stegle, Wolf Reik, Gavin Kelsey

6) Optimized cell transplantation using adult rag2 mutant zebrafish (Nature Methods) Sunday July 20th 2014
Qin Tang, Nouran S Abdelfattah, Jessica S Blackburn, John C Moore, Sarah A Martinez, Finola E Moore, Riadh Lobbardi, In, Myron S Ignatius, Jason N Berman, Robert S Liwski, Yariv Houvras, David M Langenau

7) Accelerated chromatin biochemistry using DNA-barcoded nucleosome libraries (Nature Methods) Sunday July 6th 2014
Uyen T T Nguyen, Lenka Bittova, Manuel M M, Beat Fierz, Yael David, Brian Houck-Loomis, Vanessa Feng, Geoffrey P Dann, Tom W Muir

8) Spatial control of membrane receptor function using ligand nanocalipers (Nature Methods) Sunday July 6th 2014
Alan Shaw, Vanessa Lundin, Ekaterina Petrova, Ferenc F, Erik Benson, Abdullah Al-Amin, Anna Herland, Andries Blokzijl, Bj, Ana I Teixeira

9) A method to recapitulate early embryonic spatial patterning in human embryonic stem cells (Nature Methods) Sunday June 29th 2014
Aryeh Warmflash, Benoit Sorre, Fred Etoc, Eric D Siggia, Ali H Brivanlou

10) All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins (Nature Methods) Sunday June 22nd 2014
Daniel R Hochbaum, Yongxin Zhao, Samouil L Farhi, Nathan Klapoetke, Christopher A Werley, Vikrant Kapoor, Peng Zou, Joel M Kralj, Dougal Maclaurin, Niklas Smedemark-Margulies, Jessica L Saulnier, Gabriella L Boulting, Christoph Straub, Yong Ku Cho, Michael Melkonian, Gane Ka-Shu Wong, D Jed Harrison, Venkatesh N Murthy, Bernardo L Sabatini, Edward S Boyden, Robert E Campbell, Adam E Cohen

11) Annotation of loci from genome-wide association studies using tissue-specific quantitative interaction proteomics (Nature Methods) Sunday June 22nd 2014
Alicia Lundby, Elizabeth J Rossin, Annette B Steffensen, Moshe Rav Acha, Christopher Newton-Cheh, Arne Pfeufer, Stacey N Lynch, S, S, Patrick T Ellinor, J Wouter Jukema, Stella Trompet, Ian Ford, Peter W Macfarlane, Bouwe P Krijthe, Albert Hofman, Andr, Bruno H Stricker, Hendrik M Nathoe, Wilko Spiering, Mark J Daly, Folkert W Asselbergs, Pim van der Harst, David J Milan, Paul I W de Bakker, Kasper Lage, Jesper V Olsen

12) Chemically defined generation of human cardiomyocytes (Nature Methods) Sunday June 15th 2014
Paul W Burridge, Elena Matsa, Praveen Shukla, Ziliang C Lin, Jared M Churko, Antje D Ebert, Feng Lan, Sebastian Diecke, Bruno Huber, Nicholas M Mordwinkin, Jordan R Plews, Oscar J Abilez, Bianxiao Cui, Joseph D Gold, Joseph C Wu

13) Protein delivery into live cells by incubation with an endosomolytic agent (Nature Methods) Sunday June 15th 2014
Alfredo Erazo-Oliveras, Kristina Najjar, Laila Dayani, Ting-Yi Wang, Gregory A Johnson, Jean-Philippe Pellois

14) Targeting cells with single vectors using multiple-feature Boolean logic (Nature Methods) Sunday June 8th 2014
Lief E Fenno, Joanna Mattis, Charu Ramakrishnan, Minsuk Hyun, Soo Yeun Lee, Miao He, Jason Tucciarone, Aslihan Selimbeyoglu, Andre Berndt, Logan Grosenick, Kelly A Zalocusky, Hannah Bernstein, Haley Swanson, Chelsey Perry, Ilka Diester, Frederick M Boyce, Caroline E Bass, Rachael Neve, Z Josh Huang, Karl Deisseroth

15) Single-cell western blotting (Nature Methods) Sunday June 1st 2014
Alex J Hughes, Dawn P Spelke, Zhuchen Xu, Chi-Chih Kang, David V Schaffer, Amy E Herr

16) idTracker: tracking individuals in a group by automatic identification of unmarked animals (Nature Methods) Sunday June 1st 2014
Alfonso P, Juli, Robert C Hinz, Sara Arganda, Gonzalo G de Polavieja

17) FlyMAD: rapid thermogenetic control of neuronal activity in freely walking Drosophila (Nature Methods) Sunday June 1st 2014
Daniel E Bath, John R Stowers, Dorothea H, Andreas Poehlmann, Barry J Dickson, Andrew D Straw

18) Comprehensive analysis of RNA-protein interactions by high-throughput sequencing (Nature Methods) Sunday May 18th 2014
Jacob M Tome, Abdullah Ozer, John M Pagano, Dan Gheba, Gary P Schroth, John T Lis

19) Bayesian approach to single-cell differential expression analysis (Nature Methods) Sunday May 18th 2014
Peter V Kharchenko, Lev Silberstein, David T Scadden

20) Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy (Nature Methods) Sunday May 18th 2014
Robert Prevedel, Young-Gyu Yoon, Maximilian Hoffmann, Nikita Pak, Gordon Wetzstein, Saul Kato, Tina Schr, Ramesh Raskar, Manuel Zimmer, Edward S Boyden, Alipasha Vaziri

21) Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography (Nature Methods) Sunday May 18th 2014
Yi-Wei Chang, Songye Chen, Elitza I Tocheva, Anke Treuner-Lange, Stephanie L, Lotte S, Grant J Jensen

22) Determination of damage-free crystal structure of an X-ray (Nature Methods) Sunday May 18th 2014
Kunio Hirata, Kyoko Shinzawa-Itoh, Naomine Yano, Shuhei Takemura, Koji Kato, Miki Hatanaka, Kazumasa Muramoto, Takako Kawahara, Tomitake Tsukihara, Eiki Yamashita, Kensuke Tono, Go Ueno, Takaaki Hikima, Hironori Murakami, Yuichi Inubushi, Makina Yabashi, Tetsuya Ishikawa, Masaki Yamamoto, Takashi Ogura, Hiroshi Sugimoto, Jian-Ren Shen, Shinya Yoshikawa, Hideo Ago

23) CRISPR transcriptional repression devices and layered circuits in mammalian cells (Nature Methods) Monday May 5th 2014
Samira Kiani, Jacob Beal, Mohammad R Ebrahimkhani, Jin Huh, Richard N Hall, Zhen Xie, Yinqing Li, Ron Weiss

24) Bone marrow (Nature Methods) Sunday May 4th 2014
Yu-suke Torisawa, Catherine S Spina, Tadanori Mammoto, Akiko Mammoto, James C Weaver, Tracy Tat, James J Collins, Donald E Ingber

25) Reversible protein inactivation by optogenetic trapping in cells (Nature Methods) Sunday May 4th 2014
Sangkyu Lee, Hyerim Park, Taeyoon Kyung, Na Yeon Kim, Sungsoo Kim, Jihoon Kim, Won Do Heo

26) Towards error-free profiling of immune repertoires (Nature Methods) Sunday May 4th 2014
Mikhail Shugay, Olga V Britanova, Ekaterina M Merzlyak, Maria A Turchaninova, Ilgar Z Mamedov, Timur R Tuganbaev, Dmitriy A Bolotin, Dmitry B Staroverov, Ekaterina V Putintseva, Karla Plevova, Carsten Linnemann, Dmitriy Shagin, Sarka Pospisilova, Sergey Lukyanov, Ton N Schumacher, Dmitriy M Chudakov

27) Interactive assembly algorithms for molecular cloning (Nature Methods) Monday April 28th 2014
Evan Appleton, Jenhan Tao, Traci Haddock, Douglas Densmore

28) Chronic, wireless recordings of large-scale brain activity in freely moving rhesus monkeys (Nature Methods) Monday April 28th 2014
David A Schwarz, Mikhail A Lebedev, Timothy L Hanson, Dragan F Dimitrov, Gary Lehew, Jim Meloy, Sankaranarayani Rajangam, Vivek Subramanian, Peter J Ifft, Zheng Li, Arjun Ramakrishnan, Andrew Tate, Katie Z Zhuang, Miguel A L Nicolelis

29) Olfactory exposure to males, including men, causes stress and related analgesia in rodents (Nature Methods) Monday April 28th 2014
Robert E Sorge, Loren J Martin, Kelsey A Isbester, Susana G Sotocinal, Sarah Rosen, Alexander H Tuttle, Jeffrey S Wieskopf, Erinn L Acland, Anastassia Dokova, Basil Kadoura, Philip Leger, Josiane C S Mapplebeck, Martina McPhail, Ada Delaney, Gustaf Wigerblad, Alan P Schumann, Tammie Quinn, Johannes Frasnelli, Camilla I Svensson, Wendy F Sternberg, Jeffrey S Mogil

30) Validation of noise models for single-cell transcriptomics (Nature Methods) Sunday April 20th 2014
Dominic Gr, Lennart Kester, Alexander van Oudenaarden

31) Efficient Bayesian-based multiview deconvolution (Nature Methods) Sunday April 20th 2014
Stephan Preibisch, Fernando Amat, Evangelia Stamataki, Mihail Sarov, Robert H Singer, Eugene Myers, Pavel Tomancak

32) Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas (Nature Methods) Sunday April 20th 2014
Christopher J Russo, Lori A Passmore

33) An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions (Nature Methods) Sunday April 20th 2014
Emmanuelle Tchekanda, Durga Sivanesan, Stephen W Michnick

34) Multiscale representation of genomic signals (Nature Methods) Sunday April 13th 2014
Theo A Knijnenburg, Stephen A Ramsey, Benjamin P Berman, Kathleen A Kennedy, Arian F A Smit, Lodewyk F A Wessels, Peter W Laird, Alan Aderem, Ilya Shmulevich

35) Single-molecule analysis of cell surface dynamics in Caenorhabditis elegans embryos (Nature Methods) Sunday April 13th 2014
Fran, William M McFadden, Baixue Yao, Edwin M Munro

36) Rapid adaptive optical recovery of optimal resolution over large volumes (Nature Methods) Sunday April 13th 2014
Kai Wang, Daniel E Milkie, Ankur Saxena, Peter Engerer, Thomas Misgeld, Marianne E Bronner, Jeff Mumm, Eric Betzig

37) Fluorescence nanoscopy by polarization modulation and polarization angle narrowing (Nature Methods) Sunday April 6th 2014
Nour Hafi, Matthias Grunwald, Laura S van den Heuvel, Timo Aspelmeier, Jian-Hua Chen, Marta Zagrebelsky, Ole M Sch, Claudia Steinem, Martin Korte, Axel Munk, Peter J Walla

38) Light-sheet microscopy using an Airy beam (Nature Methods) Sunday April 6th 2014
Tom Vettenburg, Heather I C Dalgarno, Jonathan Nylk, Clara Coll-Llad, David E K Ferrier, Tom, Frank J Gunn-Moore, Kishan Dholakia

39) Measuring similarity between dynamic ensembles of biomolecules (Nature Methods) Sunday April 6th 2014
Shan Yang, Lo, Hashim M Al-Hashimi

40) Quantifying Drosophila food intake: comparative analysis of current methodology (Nature Methods) Sunday March 30th 2014
Sonali A Deshpande, Gil B Carvalho, Ariadna Amador, Angela M Phillips, Sany Hoxha, Keith J Lizotte, William W Ja

41) Transcriptional profiling of cells sorted by RNA abundance (Nature Methods) Sunday March 30th 2014
Sandy Klemm, Stefan Semrau, Kay Wiebrands, Dylan Mooijman, Dina A Faddah, Rudolf Jaenisch, Alexander van Oudenaarden

42) Function-based identification of mammalian enhancers using site-specific integration (Nature Methods) Sunday March 23rd 2014
Diane E Dickel, Yiwen Zhu, Alex S Nord, John N Wylie, Jennifer A Akiyama, Veena Afzal, Ingrid Plajzer-Frick, Aileen Kirkpatrick, Berthold G, Benoit G Bruneau, Axel Visel, Len A Pennacchio

43) FIREWACh: high-throughput functional detection of transcriptional regulatory modules in mammalian cells (Nature Methods) Sunday March 23rd 2014
Matthew Murtha, Zeynep Tokcaer-Keskin, Zuojian Tang, Francesco Strino, Xi Chen, Yatong Wang, Xiangmei Xi, Claudio Basilico, Stuart Brown, Richard Bonneau, Yuval Kluger, Lisa Dailey

44) The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells (Nature Methods) Sunday March 23rd 2014
Julia Petschnigg, Bella Groisman, Max Kotlyar, Mikko Taipale, Yong Zheng, Christoph F Kurat, Azin Sayad, J Rafael Sierra, Mojca Mattiazzi Usaj, Jamie Snider, Alex Nachman, Irina Krykbaeva, Ming-Sound Tsao, Jason Moffat, Tony Pawson, Susan Lindquist, Igor Jurisica, Igor Stagljar

45) Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon (Nature Methods) Monday March 17th 2014
Christian Fr, M Wayne Davis, Mihail Sarov, Jon Taylor, Stephane Flibotte, Matthew LaBella, Andrei Pozniakovski, Donald G Moerman, Erik M Jorgensen

46) Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein (Nature Methods) Monday March 17th 2014
Jun Chu, Russell D Haynes, St, Pengpeng Li, Emilio Gonz, John S Burg, Niloufar J Ataie, Amy J Lam, Paula J Cranfill, Michelle A Baird, Michael W Davidson, Ho-Leung Ng, K Christopher Garcia, Christopher H Contag, Kang Shen, Helen M Blau, Michael Z Lin

47) Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers (Nature Methods) Monday March 17th 2014
Johan Hattne, Nathaniel Echols, Rosalie Tran, Jan Kern, Richard J Gildea, Aaron S Brewster, Roberto Alonso-Mori, Carina Gl, Julia Hellmich, Hartawan Laksmono, Raymond G Sierra, Benedikt Lassalle-Kaiser, Alyssa Lampe, Guangye Han, Sheraz Gul, D, Despina Milathianaki, Alan R Fry, Alan Miahnahri, William E White, Donald W Schafer, M Marvin Seibert, Jason E Koglin, Dimosthenis Sokaras, Tsu-Chien Weng, Jonas Sellberg, Matthew J Latimer, Pieter Glatzel, Petrus H Zwart, Ralf W Grosse-Kunstleve, Michael J Bogan, Marc Messerschmidt, Garth J Williams, S, Johannes Messinger, Athina Zouni, Junko Yano, Uwe Bergmann, Vittal K Yachandra, Paul D Adams, Nicholas K Sauter

48) PyClone: statistical inference of clonal population structure in cancer (Nature Methods) Monday March 17th 2014
Andrew Roth, Jaswinder Khattra, Damian Yap, Adrian Wan, Emma Laks, Justina Biele, Gavin Ha, Samuel Aparicio, Alexandre Bouchard-C, Sohrab P Shah

49) Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals (Nature Methods) Sunday March 9th 2014
Evan J Olson, Lucas A Hartsough, Brian P Landry, Raghav Shroff, Jeffrey J Tabor

50) Dynamic characterization of growth and gene expression using high-throughput automated flow cytometry (Nature Methods) Sunday March 9th 2014
Ignacio A Zuleta, Andr, Hao Li, Hana El-Samad

51) Single-molecule motions enable direct visualization of biomolecular interactions in solution (Nature Methods) Sunday March 9th 2014
Quan Wang, W E Moerner

52) Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry (Nature Methods) Sunday March 2nd 2014
Charlotte Giesen, Hao A O Wang, Denis Schapiro, Nevena Zivanovic, Andrea Jacobs, Bodo Hattendorf, Peter J Sch, Daniel Grolimund, Joachim M Buhmann, Simone Brandt, Zsuzsanna Varga, Peter J Wild, Detlef G, Bernd Bodenmiller

53) Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects (Nature Methods) Sunday March 2nd 2014
Bin Shen, Wensheng Zhang, Jun Zhang, Jiankui Zhou, Jianying Wang, Li Chen, Lu Wang, Alex Hodgkins, Vivek Iyer, Xingxu Huang, William C Skarnes

54) Structure determination of noncanonical RNA motifs guided by 1H NMR chemical shifts (Nature Methods) Sunday March 2nd 2014
Parin Sripakdeevong, Mirko Cevec, Andrew T Chang, Mich, Melanie Ziegeler, Qin Zhao, George E Fox, Xiaolian Gao, Scott D Kennedy, Ryszard Kierzek, Edward P Nikonowicz, Harald Schwalbe, Roland K O Sigel, Douglas H Turner, Rhiju Das

55) Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering (Nature Methods) Sunday March 2nd 2014
Lu Wei, Fanghao Hu, Yihui Shen, Zhixing Chen, Yong Yu, Chih-Chun Lin, Meng C Wang, Wei Min

56) Sleep-spindle detection: crowdsourcing and evaluating performance of experts, non-experts and automated methods (Nature Methods) Sunday February 23rd 2014
Simon C Warby, Sabrina L Wendt, Peter Welinder, Emil G S Munk, Oscar Carrillo, Helge B D Sorensen, Poul Jennum, Paul E Peppard, Pietro Perona, Emmanuel Mignot

57) Gold rotor bead tracking for high-speed measurements of DNA twist, torque and extension (Nature Methods) Sunday February 23rd 2014
Paul Lebel, Aakash Basu, Florian C Oberstrass, Elsa M Tretter, Zev Bryant

58) Identification of small molecules that support human leukemia stem cell activity ex vivo (Nature Methods) Sunday February 23rd 2014
Caroline Pabst, Jana Krosl, Iman Fares, Genevi, R, Anne Marinier, S, Jos, Guy Sauvageau

59) Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity (Nature Methods) Sunday February 16th 2014
John P Guilinger, Vikram Pattanayak, Deepak Reyon, Shengdar Q Tsai, Jeffry D Sander, J Keith Joung, David R Liu

60) Cryo-scanning transmission electron tomography of vitrified cells (Nature Methods) Sunday February 16th 2014
Sharon Grayer Wolf, Lothar Houben, Michael Elbaum

61) Efficient multivariate linear mixed model algorithms for genome-wide association studies (Nature Methods) Sunday February 16th 2014
Xiang Zhou, Matthew Stephens

62) Independent optical excitation of distinct neural populations (Nature Methods) Sunday February 9th 2014
Nathan C Klapoetke, Yasunobu Murata, Sung Soo Kim, Stefan R Pulver, Amanda Birdsey-Benson, Yong Ku Cho, Tania K Morimoto, Amy S Chuong, Eric J Carpenter, Zhijian Tian, Jun Wang, Yinlong Xie, Zhixiang Yan, Yong Zhang, Brian Y Chow, Barbara Surek, Michael Melkonian, Vivek Jayaraman, Martha Constantine-Paton, Gane Ka-Shu Wong, Edward S Boyden

63) A synthetic luciferin improves bioluminescence imaging in live mice (Nature Methods) Sunday February 9th 2014
Melanie S Evans, Joanna P Chaurette, Spencer T Adams, Gadarla R Reddy, Miranwda A Paley, Neil Aronin, Jennifer A Prescher, Stephen C Miller

64) Isolation of single-base genome-edited human iPS cells without antibiotic selection (Nature Methods) Sunday February 9th 2014
Yuichiro Miyaoka, Amanda H Chan, Luke M Judge, Jennie Yoo, Miller Huang, Trieu D Nguyen, Paweena P Lizarraga, Po-Lin So, Bruce R Conklin

65) A mass spectrometry (Nature Methods) Sunday February 9th 2014
Argyris Politis, Florian Stengel, Zoe Hall, Helena Hern, Alexander Leitner, Thomas Walzthoeni, Carol V Robinson, Ruedi Aebersold

66) Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT (Nature Methods) Sunday February 2nd 2014
Ralf Jungmann, Maier S Avenda, Johannes B Woehrstein, Mingjie Dai, William M Shih, Peng Yin

67) Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells (Nature Methods) Sunday February 2nd 2014
Nils A Kulak, Garwin Pichler, Igor Paron, Nagarjuna Nagaraj, Matthias Mann

68) Functional annotation of noncoding sequence variants (Nature Methods) Sunday February 2nd 2014
Graham R S Ritchie, Ian Dunham, Eleftheria Zeggini, Paul Flicek

69) Similarity network fusion for aggregating data types on a genomic scale (Nature Methods) Sunday January 26th 2014
Bo Wang, Aziz M Mezlini, Feyyaz Demir, Marc Fiume, Zhuowen Tu, Michael Brudno, Benjamin Haibe-Kains, Anna Goldenberg

70) Correlative super-resolution fluorescence and metal-replica transmission electron microscopy (Nature Methods) Sunday January 26th 2014
Kem A Sochacki, Gleb Shtengel, Schuyler B van Engelenburg, Harald F Hess, Justin W Taraska

71) Epigenome-wide association studies without the need for cell-type composition (Nature Methods) Sunday January 26th 2014
James Zou, Christoph Lippert, David Heckerman, Martin Aryee, Jennifer Listgarten

72) Objective comparison of particle tracking methods (Nature Methods) Sunday January 19th 2014
Nicolas Chenouard, Ihor Smal, Fabrice de Chaumont, Martin Ma, Ivo F Sbalzarini, Yuanhao Gong, Janick Cardinale, Craig Carthel, Stefano Coraluppi, Mark Winter, Andrew R Cohen, William J Godinez, Karl Rohr, Yannis Kalaidzidis, Liang Liang, James Duncan, Hongying Shen, Yingke Xu, Klas E G Magnusson, Joakim Jald, Helen M Blau, Perrine Paul-Gilloteaux, Philippe Roudot, Charles Kervrann, Fran, Jean-Yves Tinevez, Spencer L Shorte, Joost Willemse, Katherine Celler, Gilles P van Wezel, Han-Wei Dan, Yuh-Show Tsai, Carlos Ortiz de Sol, Jean-Christophe Olivo-Marin, Erik Meijering

73) Targeted protein quantification using sparse reference labeling (Nature Methods) Sunday January 19th 2014
Ching-Yun Chang, Eduard Sabid, Ruedi Aebersold, Olga Vitek

74) Continuous throughput and long-term observation of single-molecule FRET without immobilization (Nature Methods) Sunday January 19th 2014
Swati Tyagi, Virginia VanDelinder, Niccol, Gustavo Fuertes, Sigrid Milles, Morgane Agez, Edward A Lemke

75) Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue (Nature Methods) Monday January 13th 2014
Ditte Lovatt, Brittani K Ruble, Jaehee Lee, Hannah Dueck, Tae Kyung Kim, Stephen Fisher, Chantal Francis, Jennifer M Spaethling, John A Wolf, M Sean Grady, Alexandra V Ulyanova, Sean B Yeldell, Julianne C Griepenburg, Peter T Buckley, Junhyong Kim, Jai-Yoon Sul, Ivan J Dmochowski, James Eberwine

76) Scalable inference of heterogeneous reaction kinetics from pooled single-cell recordings (Nature Methods) Monday January 13th 2014
Christoph Zechner, Michael Unger, Serge Pelet, Matthias Peter, Heinz Koeppl

77) EC-BLAST: a tool to automatically search and compare enzyme reactions (Nature Methods) Monday January 13th 2014
Syed Asad Rahman, Sergio Martinez Cuesta, Nicholas Furnham, Gemma L Holliday, Janet M Thornton

78) Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate (Nature Methods) Sunday January 5th 2014
Nela Durisic, Lara Laparra-Cuervo, Ángel Sandoval-Álvarez, Joseph Steven Borbely, Melike Lakadamyali

79) Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes (Nature Methods) Sunday January 5th 2014
Thomas Thestrup, Julia Litzlbauer, Ingo Bartholom, Marsilius Mues, Luigi Russo, Hod Dana, Yuri Kovalchuk, Yajie Liang, Georgios Kalamakis, Yvonne Laukat, Stefan Becker, Gregor Witte, Anselm Geiger, Taylor Allen, Lawrence C Rome, Tsai-Wen Chen, Douglas S Kim, Olga Garaschuk, Christian Griesinger, Oliver Griesbeck

80) Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship (Nature Methods) Sunday December 22nd 2013
Hidehiko K Inagaki, Yonil Jung, Eric D Hoopfer, Allan M Wong, Neeli Mishra, John Y Lin, Roger Y Tsien, David J Anderson

81) Quantitative single-cell RNA-seq with unique molecular identifiers (Nature Methods) Sunday December 22nd 2013
Saiful Islam, Amit Zeisel, Simon Joost, Gioele La Manno, Pawel Zajac, Maria Kasper, Peter L, Sten Linnarsson

82) High-resolution mapping of transcription factor binding sites on native chromatin (Nature Methods) Sunday December 15th 2013
Sivakanthan Kasinathan, Guillermo A Orsi, Gabriel E Zentner, Kami Ahmad, Steven Henikoff

83) Drift time-specific collision energies enable deep-coverage data- independent acquisition proteomics (Nature Methods) Sunday December 15th 2013
Ute Distler, J, Pedro Navarro, Yishai Levin, Hansj, Stefan Tenzer

84) Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins (Nature Methods) Monday December 9th 2013
Jacob J Kennedy, Susan E Abbatiello, Kyunggon Kim, Ping Yan, Jeffrey R Whiteaker, Chenwei Lin, Jun Seok Kim, Yuzheng Zhang, Xianlong Wang, Richard G Ivey, Lei Zhao, Hophil Min, Youngju Lee, Myeong-Hee Yu, Eun Gyeong Yang, Cheolju Lee, Pei Wang, Henry Rodriguez, Youngsoo Kim, Steven A Carr, Amanda G Paulovich

85) A chemoproteomic platform to quantitatively map targets of lipid-derived electrophiles (Nature Methods) Sunday December 1st 2013
Chu Wang, Eranthie Weerapana, Megan M Blewett, Benjamin F Cravatt

86) Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny (Nature Methods) Sunday December 1st 2013
Xiaolei Yin, Henner F Farin, Johan H van Es, Hans Clevers, Robert Langer, Jeffrey M Karp

87) A quantitative liposome microarray to systematically characterize protein-lipid interactions (Nature Methods) Sunday November 24th 2013
Antoine-Emmanuel Saliba, Ivana Vonkova, Stefano Ceschia, Greg M Findlay, Kenji Maeda, Christian Tischer, Samy Deghou, Vera van Noort, Peer Bork, Tony Pawson, Jan Ellenberg, Anne-Claude Gavin

88) Quantifying RNA allelic ratios by microfluidic multiplex PCR and sequencing (Nature Methods) Sunday November 24th 2013
Rui Zhang, Xin Li, Gokul Ramaswami, Kevin S Smith, Gustavo Turecki, Stephen B Montgomery, Jin Billy Li

89) A holidic medium for Drosophila melanogaster (Nature Methods) Sunday November 17th 2013
Matthew D W Piper, Eric Blanc, Ricardo Leit, Mingyao Yang, Xiaoli He, Nancy J Linford, Matthew P Hoddinott, Corinna Hopfen, George A Soultoukis, Christine Niemeyer, Fiona Kerr, Scott D Pletcher, Carlos Ribeiro, Linda Partridge

90) Integrating protein-protein interaction networks with phenotypes reveals signs of interactions (Nature Methods) Sunday November 17th 2013
Arunachalam Vinayagam, Jonathan Zirin, Charles Roesel, Yanhui Hu, Bahar Yilmazel, Anastasia A Samsonova, Ralph A Neum, Stephanie E Mohr, Norbert Perrimon

91) HiRIEF LC-MS enables deep proteome coverage and unbiased proteogenomics (Nature Methods) Sunday November 17th 2013
Rui M M Branca, Lukas M Orre, Henrik J Johansson, Viktor Granholm, Mikael Huss, Åsa Pérez-Bercoff, Jenny Forshed, Lukas K, Janne Lehti

92) Deciphering laminar-specific neural inputs with line-scanning fMRI (Nature Methods) Sunday November 17th 2013
Xin Yu, Chunqi Qian, Der-yow Chen, Stephen J Dodd, Alan P Koretsky

93) Parallel measurement of dynamic changes in translation rates in single cells (Nature Methods) Sunday November 10th 2013
Kyuho Han, Ariel Jaimovich, Gautam Dey, Davide Ruggero, Oded Meyuhas, Nahum Sonenberg, Tobias Meyer

94) Quantifying the local resolution of cryo-EM density maps (Nature Methods) Sunday November 10th 2013
Alp Kucukelbir, Fred J Sigworth, Hemant D Tagare

95) Systematic evaluation of spliced alignment programs for RNA-seq data (Nature Methods) Sunday November 3rd 2013
P, Tamara Steijger, Botond Sipos, Gregory R Grant, Andr, Gunnar R, Nick Goldman, Tim J Hubbard, Jennifer Harrow, Roderic Guig, Paul Bertone

96) Assessment of transcript reconstruction methods for RNA-seq (Nature Methods) Sunday November 3rd 2013
Tamara Steijger, Josep F Abril, P, Felix Kokocinski, Tim J Hubbard, Roderic Guig, Jennifer Harrow, Paul Bertone

97) Coupling transcription factor occupancy to nucleosome architecture with DNase-FLASH (Nature Methods) Sunday November 3rd 2013
Jeff Vierstra, Hao Wang, Sam John, Richard Sandstrom, John A Stamatoyannopoulos

98) Accelerated discovery via a whole-cell model (Nature Methods) Sunday November 3rd 2013
Jayodita C Sanghvi, Sergi Regot, Silvia Carrasco, Jonathan R Karr, Miriam V Gutschow, Benjamin Bolival, Markus W Covert

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