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1) CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo (Nature Methods) Monday August 31st 2015
Miguel A Moreno-Mateos, Charles E Vejnar, Jean-Denis Beaudoin, Juan P Fernandez, Emily K Mis, Mustafa K Khokha, Antonio J Giraldez

2) Programmed synthesis of three-dimensional tissues (Nature Methods) Monday August 31st 2015
Michael E Todhunter, Noel Y Jee, Alex J Hughes, Maxwell C Coyle, Alec Cerchiari, Justin Farlow, James C Garbe, Mark A LaBarge, Tejal A Desai, Zev J Gartner

3) Inntags: small self-structured epitopes for innocuous protein tagging (Nature Methods) Monday August 31st 2015
Maya V Georgieva, Galal Yahya, Laia Cod, Ra, Laura Teixid, Jos, Ricardo Jara, M, Antoni Iborra, Josep Llu, Carme Gallego, Modesto Orozco, Mart

4) Wirelessly powered, fully internal optogenetics for brain, spinal and peripheral circuits in mice (Nature Methods) Monday August 24th 2015
Kate L Montgomery, Alexander J Yeh, John S Ho, Vivien Tsao, Shrivats Mohan Iyer, Logan Grosenick, Emily A Ferenczi, Yuji Tanabe, Karl Deisseroth, Scott L Delp, Ada S Y Poon

5) High-throughput cellular RNA device engineering (Nature Methods) Monday August 24th 2015
Brent Townshend, Andrew B Kennedy, Joy S Xiang, Christina D Smolke

6) Oscope identifies oscillatory genes in unsynchronized single-cell RNA-seq experiments (Nature Methods) Monday August 24th 2015
Ning Leng, Li-Fang Chu, Chris Barry, Yuan Li, Jeea Choi, Xiaomao Li, Peng Jiang, Ron M Stewart, James A Thomson, Christina Kendziorski

7) Predicting effects of noncoding variants with deep learning (Nature Methods) Monday August 24th 2015
Jian Zhou, Olga G Troyanskaya

8) EMRinger: side chain (Nature Methods) Monday August 24th 2015
Benjamin A Barad, Nathaniel Echols, Ray Yu-Ruei Wang, Yifan Cheng, Frank DiMaio, Paul D Adams, James S Fraser

9) ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors (Nature Methods) Monday August 24th 2015
Christian Schmidl, Andr, Nathan C Sheffield, Christoph Bock

10) Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy (Nature Methods) Monday August 24th 2015
Zhengyang Zhang, Samuel J Kenny, Margaret Hauser, Wan Li, Ke Xu

11) SpeedSeq: ultra-fast personal genome analysis and interpretation (Nature Methods) Monday August 24th 2015
Colby Chiang, Ryan M Layer, Gregory G Faust, Michael R Lindberg, David B Rose, Erik P Garrison, Gabor T Marth, Aaron R Quinlan, Ira M Hall

12) Continuous directed evolution of DNA-binding proteins to improve TALEN specificity (Nature Methods) Monday August 24th 2015
Basil P Hubbard, Ahmed H Badran, John A Zuris, John P Guilinger, Kevin M Davis, Liwei Chen, Shengdar Q Tsai, Jeffry D Sander, J Keith Joung, David R Liu

13) ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space (Nature Methods) Monday August 3rd 2015
Ashwin Chari, David Haselbach, Jan-Martin Kirves, Juergen Ohmer, Elham Paknia, Niels Fischer, Oleg Ganichkin, Vanessa M, Jeremiah J Frye, Georg Petzold, Marc Jarvis, Michael Tietzel, Clemens Grimm, Jan-Michael Peters, Brenda A Schulman, Kai Tittmann, J, Utz Fischer, Holger Stark

14) ARM-seq: AlkB-facilitated RNA methylation sequencing reveals a complex landscape of modified tRNA fragments (Nature Methods) Monday August 3rd 2015
Aaron E Cozen, Erin Quartley, Andrew D Holmes, Eva Hrabeta-Robinson, Eric M Phizicky, Todd M Lowe

15) Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells (Nature Methods) Monday August 3rd 2015
Daniel Paull, Ana Sevilla, Hongyan Zhou, Aana Kim Hahn, Hesed Kim, Christopher Napolitano, Alexander Tsankov, Linshan Shang, Katie Krumholz, Premlatha Jagadeesan, Chris M Woodard, Bruce Sun, Thierry Vilboux, Matthew Zimmer, Eliana Forero, Dorota N Moroziewicz, Hector Martinez, May Christine V Malicdan, Keren A Weiss, Lauren B Vensand, Carmen R Dusenberry, Hannah Polus, Karla Therese L Sy, David J Kahler, William A Gahl, Susan L Solomon, Stephen Chang, Alexander Meissner, Kevin Eggan, Scott A Noggle

16) Mutational interference mapping experiment (MIME) for studying RNA structure and function (Nature Methods) Monday August 3rd 2015
Redmond P Smyth, Laurence Despons, Gong Huili, Serena Bernacchi, Marcel Hijnen, Johnson Mak, Fabrice Jossinet, Li Weixi, Jean-Christophe Paillart, Max von Kleist, Roland Marquet

17) EEG and functional ultrasound imaging in mobile rats (Nature Methods) Monday August 3rd 2015
Lim-Anna Sieu, Antoine Bergel, Elodie Tiran, Thomas Deffieux, Mathieu Pernot, Jean-Luc Gennisson, Micka, Ivan Cohen

18) Real-time imaging of brain activity in freely moving rats using functional ultrasound (Nature Methods) Monday July 27th 2015
Alan Urban, Clara Dussaux, Guillaume Martel, Cl, Emilie Mace, Gabriel Montaldo

19) Imaging G protein (Nature Methods) Monday July 27th 2015
David Alsteens, Moritz Pfreundschuh, Cheng Zhang, Patrizia M Spoerri, Shaun R Coughlin, Brian K Kobilka, Daniel J M

20) Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging (Nature Methods) Monday July 27th 2015
Shai Berlin, Elizabeth C Carroll, Zachary L Newman, Hitomi O Okada, Carson M Quinn, Benjamin Kallman, Nathan C Rockwell, Shelley S Martin, J Clark Lagarias, Ehud Y Isacoff

21) A microfluidic device for epigenomic profiling using 100 cells (Nature Methods) Monday July 27th 2015
Zhenning Cao, Changya Chen, Bing He, Kai Tan, Chang Lu

22) Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics (Nature Methods) Monday July 27th 2015
Jale Schneider, Jasmin Zahn, Marta Maglione, Stephan J Sigrist, Jonas Marquard, Jakub Chojnacki, Hans-Georg Kr, Steffen J Sahl, Johann Engelhardt, Stefan W Hell

23) Efficient and quantitative high-throughput tRNA sequencing (Nature Methods) Monday July 27th 2015
Guanqun Zheng, Yidan Qin, Wesley C Clark, Qing Dai, Chengqi Yi, Chuan He, Alan M Lambowitz, Tao Pan

24) Inferring transient particle transport dynamics in live cells (Nature Methods) Monday July 27th 2015
Nilah Monnier, Zachary Barry, Hye Yoon Park, Kuan-Chung Su, Zachary Katz, Brian P English, Arkajit Dey, Keyao Pan, Iain M Cheeseman, Robert H Singer, Mark Bathe

25) Phenolyzer: phenotype-based prioritization of candidate genes for human diseases (Nature Methods) Monday July 27th 2015
Hui Yang, Peter N Robinson, Kai Wang

26) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach (Nature Methods) Monday July 27th 2015
Raj Chari, Prashant Mali, Mark Moosburner, George M Church

27) Continuous volumetric imaging via an optical phase-locked ultrasound lens (Nature Methods) Monday July 27th 2015
Lingjie Kong, Jianyong Tang, Justin P Little, Yang Yu, Tim L, Charles P Lin, Ronald N Germain, Meng Cui

28) Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells (Nature Methods) Monday July 6th 2015
Iwo K, Arash Zarrine-Afsar, Mikayel Aznauryan, Andrea Soranno, Bengt Wunderlich, Fabian Dingfelder, Jakob C St, Andreas Pl, Daniel Nettels, Benjamin Schuler

29) Multitarget super-resolution microscopy with high-density labeling by exchangeable probes (Nature Methods) Monday July 6th 2015
Tai Kiuchi, Makio Higuchi, Akihiro Takamura, Masahiro Maruoka, Naoki Watanabe

30) Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation (Nature Methods) Monday June 29th 2015
Edyta Marcon, Harshika Jain, Anandi Bhattacharya, Hongbo Guo, Sadhna Phanse, Shuye Pu, Gregory Byram, Ben C Collins, Evan Dowdell, Maria Fenner, Xinghua Guo, Ashley Hutchinson, Jacob J Kennedy, Bryan Krastins, Brett Larsen, Zhen-Yuan Lin, Mary F Lopez, Peter Loppnau, Shane Miersch, Tin Nguyen, Jonathan B Olsen, Marcin Paduch, Mani Ravichandran, Alma Seitova, Gouri Vadali, Maryann S Vogelsang, Jeffrey R Whiteaker, Guoqing Zhong, Nan Zhong, Lei Zhao, Ruedi Aebersold, Cheryl H Arrowsmith, Andrew Emili, Lori Frappier, Anne-Claude Gingras, Matthias Gstaiger, Amanda G Paulovich, Shohei Koide, Anthony A Kossiakoff, Sachdev S Sidhu, Shoshana J Wodak, Susanne Gr, Jack F Greenblatt, Aled M Edwards

31) Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome (Nature Methods) Monday June 29th 2015
Bastian Linder, Anya V Grozhik, Anthony O Olarerin-George, Cem Meydan, Christopher E Mason, Samie R Jaffrey

32) Assembly and diploid architecture of an individual human genome via single-molecule technologies (Nature Methods) Monday June 29th 2015
Matthew Pendleton, Robert Sebra, Andy Wing Chun Pang, Ajay Ummat, Oscar Franzen, Tobias Rausch, Adrian M St, William Stedman, Thomas Anantharaman, Alex Hastie, Heng Dai, Markus Hsi-Yang Fritz, Han Cao, Ariella Cohain, Gintaras Deikus, Russell E Durrett, Scott C Blanchard, Roger Altman, Chen-Shan Chin, Yan Guo, Ellen E Paxinos, Jan O Korbel, Robert B Darnell, W Richard McCombie, Pui-Yan Kwok, Christopher E Mason, Eric E Schadt, Ali Bashir

33) Protein structure determination by combining sparse NMR data with evolutionary couplings (Nature Methods) Monday June 29th 2015
Yuefeng Tang, Yuanpeng Janet Huang, Thomas A Hopf, Chris Sander, Debora S Marks, Gaetano T Montelione

34) Single-cell magnetic imaging using a quantum diamond microscope (Nature Methods) Monday June 22nd 2015
David R Glenn, Kyungheon Lee, Hongkun Park, Ralph Weissleder, Amir Yacoby, Mikhail D Lukin, Hakho Lee, Ronald L Walsworth, Colin B Connolly

35) Combining protein and mRNA quantification to decipher transcriptional regulation (Nature Methods) Monday June 22nd 2015
Heng Xu, Leonardo A Sep, Lauren Figard, Anna Marie Sokac, Ido Golding

36) A naturally monomeric infrared fluorescent protein for protein labeling in vivo (Nature Methods) Monday June 22nd 2015
Dan Yu, Michelle A Baird, John R Allen, Elizabeth S Howe, Matthew P Klassen, Anna Reade, Kalpana Makhijani, Yuanquan Song, Songmei Liu, Zehra Murthy, Shao-Qing Zhang, Orion D Weiner, Thomas B Kornberg, Yuh-Nung Jan, Michael W Davidson, Xiaokun Shu

37) Quantitative evaluation of software packages for single-molecule localization microscopy (Nature Methods) Monday June 15th 2015
Daniel Sage, Hagai Kirshner, Thomas Pengo, Nico Stuurman, Junhong Min, Suliana Manley, Michael Unser

38) Efficient set tests for the genetic analysis of correlated traits (Nature Methods) Monday June 15th 2015
Francesco Paolo Casale, Barbara Rakitsch, Christoph Lippert, Oliver Stegle

39) A complete bacterial genome assembled de novo using only nanopore sequencing data (Nature Methods) Monday June 15th 2015
Nicholas J Loman, Joshua Quick, Jared T Simpson

40) Quantifying domain-ligand affinities and specificities by high-throughput holdup assay (Nature Methods) Monday June 8th 2015
Renaud Vincentelli, Katja Luck, Juline Poirson, Jolanta Polanowska, Julie Abdat, Marilyne Bl, Jeremy Turchetto, Fran, Kevin Ricquier, Marie-Laure Straub, Anne Forster, Patricia Cassonnet, Jean-Paul Borg, Yves Jacob, Murielle Masson, Yves Nomin, J, Nicolas Wolff, Sebastian Charbonnier, Gilles Trav

41) Homology modeling of larger proteins guided by chemical shifts (Nature Methods) Monday June 8th 2015
Yang Shen, Ad Bax

42) Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display (Nature Methods) Monday June 1st 2015
David M Shechner, Ezgi Hacisuleyman, Scott T Younger, John L Rinn

43) Functional differentiation of human pluripotent stem cells on a chip (Nature Methods) Monday June 1st 2015
Giovanni G Giobbe, Federica Michielin, Camilla Luni, Stefano Giulitti, Sebastian Martewicz, Sirio Dupont, Annarosa Floreani, Nicola Elvassore

44) Traction microscopy to identify force modulation in subresolution adhesions (Nature Methods) Monday June 1st 2015
Sangyoon J Han, Youbean Oak, Alex Groisman, Gaudenz Danuser

45) Application of BRET to monitor ligand binding to GPCRs (Nature Methods) Monday June 1st 2015
Leigh A Stoddart, Elizabeth K M Johnstone, Amanda J Wheal, Jo, Matthew B Robers, Thomas Machleidt, Keith V Wood, Stephen J Hill, Kevin D G Pfleger

46) Plasmodium falciparum genetic crosses in a humanized mouse model (Nature Methods) Monday June 1st 2015
Ashley M Vaughan, Richard S Pinapati, Ian H Cheeseman, Nelly Camargo, Matthew Fishbaugher, Lisa A Checkley, Shalini Nair, Carolyn A Hutyra, Fran, Timothy J C Anderson, Michael T Ferdig, Stefan H I Kappe

47) A microfluidic device for label-free, physical capture of circulating tumor cell clusters (Nature Methods) Monday May 25th 2015
A Fatih Sarioglu, Nicola Aceto, Nikola Kojic, Maria C Donaldson, Mahnaz Zeinali, Bashar Hamza, Amanda Engstrom, Huili Zhu, Tilak K Sundaresan, David T Miyamoto, Xi Luo, Aditya Bardia, Ben S Wittner, Sridhar Ramaswamy, Toshi Shioda, David T Ting, Shannon L Stott, Ravi Kapur, Shyamala Maheswaran, Daniel A Haber, Mehmet Toner

48) Automated determination of fibrillar structures by simultaneous model building and fiber diffraction refinement (Nature Methods) Monday May 25th 2015
Wojciech Potrzebowski, Ingemar Andr

49) Rapid, optimized interactomic screening (Nature Methods) Monday May 25th 2015
Zhanna Hakhverdyan, Michal Domanski, Loren E Hough, Asha A Oroskar, Anil R Oroskar, Sarah Keegan, David J Dilworth, Kelly R Molloy, Vadim Sherman, John D Aitchison, David Feny, Brian T Chait, Torben Heick Jensen, Michael P Rout, John LaCava

50) Dexterous robotic manipulation of alert adult Drosophila for high-content experimentation (Nature Methods) Monday May 25th 2015
Joan Savall, Eric Tatt Wei Ho, Cheng Huang, Jessica R Maxey, Mark J Schnitzer

51) In vivo single-cell labeling by confined primed conversion (Nature Methods) Monday May 25th 2015
William P Dempsey, Lada Georgieva, Patrick M Helbling, Ali Y Sonay, Thai V Truong, Michel Haffner, Periklis Pantazis

52) Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR (Nature Methods) Monday May 25th 2015
Mohammed Kaplan, Abhishek Cukkemane, Gydo C P van Zundert, Siddarth Narasimhan, Mark Dani, Deni Mance, Gabriel Waksman, Alexandre M J J Bonvin, R, Gert E Folkers, Marc Baldus

53) 3D high- and super-resolution imaging using single-objective SPIM (Nature Methods) Monday May 25th 2015
Remi Galland, Gianluca Grenci, Ajay Aravind, Virgile Viasnoff, Vincent Studer, Jean-Baptiste Sibarita

54) Structural analysis of multicellular organisms with cryo-electron tomography (Nature Methods) Monday May 25th 2015
Jan Harapin, Mandy B, K Tanuj Sapra, Damian Brunner, Andres Kaech, Ohad Medalia

55) CONSERTING: integrating copy-number analysis with structural-variation detection (Nature Methods) Monday May 25th 2015
Xiang Chen, Pankaj Gupta, Jianmin Wang, Joy Nakitandwe, Kathryn Roberts, James D Dalton, Matthew Parker, Samir Patel, Linda Holmfeldt, Debbie Payne, John Easton, Jing Ma, Michael Rusch, Gang Wu, Aman Patel, Suzanne J Baker, Michael A Dyer, Sheila Shurtleff, Stephen Espy, Stanley Pounds, James R Downing, David W Ellison, Charles G Mullighan, Jinghui Zhang

56) MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis (Nature Methods) Monday May 25th 2015
Hiroshi Tsugawa, Tomas Cajka, Tobias Kind, Yan Ma, Brendan Higgins, Kazutaka Ikeda, Mitsuhiro Kanazawa, Jean VanderGheynst, Oliver Fiehn, Masanori Arita

57) MIMP: predicting the impact of mutations on kinase-substrate phosphorylation (Nature Methods) Monday May 25th 2015
Omar Wagih, J, Gary D Bader

58) Multispectral labeling technique to map many neighboring axonal projections in the same tissue (Nature Methods) Monday April 27th 2015
Shlomo Tsuriel, Sagi Gudes, Ryan W Draft, Alexander M Binshtok, Jeff W Lichtman

59) High-performance probes for light and electron microscopy (Nature Methods) Monday April 27th 2015
Sarada Viswanathan, Megan E Williams, Erik B Bloss, Timothy J Stasevich, Colenso M Speer, Aljoscha Nern, Barret D Pfeiffer, Bryan M Hooks, Wei-Ping Li, Brian P English, Teresa Tian, Gilbert L Henry, John J Macklin, Ronak Patel, Charles R Gerfen, Xiaowei Zhuang, Yalin Wang, Gerald M Rubin, Loren L Looger

60) Isotope-targeted glycoproteomics (IsoTaG): a mass-independent platform for intact N- and O-glycopeptide discovery and analysis (Nature Methods) Monday April 27th 2015
Christina M Woo, Anthony T Iavarone, David R Spiciarich, Krishnan K Palaniappan, Carolyn R Bertozzi

61) Gamp;T-seq: parallel sequencing of single-cell genomes and transcriptomes (Nature Methods) Monday April 27th 2015
Iain C Macaulay, Wilfried Haerty, Parveen Kumar, Yang I Li, Tim Xiaoming Hu, Mabel J Teng, Mubeen Goolam, Nathalie Saurat, Paul Coupland, Lesley M Shirley, Miriam Smith, Niels Van der Aa, Ruby Banerjee, Peter D Ellis, Michael A Quail, Harold P Swerdlow, Magdalena Zernicka-Goetz, Frederick J Livesey, Chris P Ponting, Thierry Voet

62) A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy (Nature Methods) Monday April 27th 2015
Dhermendra K Tiwari, Yoshiyuki Arai, Masahito Yamanaka, Tomoki Matsuda, Masakazu Agetsuma, Masahiro Nakano, Katsumasa Fujita, Takeharu Nagai

63) In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy (Nature Methods) Monday April 13th 2015
Deepak R Chittajallu, Stefan Florian, Rainer H Kohler, Yoshiko Iwamoto, James D Orth, Ralph Weissleder, Gaudenz Danuser, Timothy J Mitchison

64) High-resolution whole-brain staining for electron microscopic circuit reconstruction (Nature Methods) Monday April 13th 2015
Shawn Mikula, Winfried Denk

65) Rapid reverse genetic screening using CRISPR in zebrafish (Nature Methods) Monday April 13th 2015
Arish N Shah, Crystal F Davey, Alex C Whitebirch, Adam C Miller, Cecilia B Moens

66) Massively parallel delivery of large cargo into mammalian cells with light pulses (Nature Methods) Monday April 6th 2015
Yi-Chien Wu, Ting-Hsiang Wu, Daniel L Clemens, Bai-Yu Lee, Ximiao Wen, Marcus A Horwitz, Michael A Teitell, Pei-Yu Chiou

67) Robust enumeration of cell subsets from tissue expression profiles (Nature Methods) Monday April 6th 2015
Aaron M Newman, Chih Long Liu, Michael R Green, Andrew J Gentles, Weiguo Feng, Yue Xu, Chuong D Hoang, Maximilian Diehn, Ash A Alizadeh

68) Quantifying cellular capacity identifies gene expression designs with reduced burden (Nature Methods) Monday April 6th 2015
Francesca Ceroni, Rhys Algar, Guy-Bart Stan, Tom Ellis

69) Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra (Nature Methods) Monday April 6th 2015
Daniel Franke, Cy M Jeffries, Dmitri I Svergun

70) Sequencing small genomic targets with high efficiency and extreme accuracy (Nature Methods) Monday April 6th 2015
Michael W Schmitt, Edward J Fox, Marc J Prindle, Kate S Reid-Bayliss, Lawrence D True, Jerald P Radich, Lawrence A Loeb

71) High-speed label-free functional photoacoustic microscopy of mouse brain in action (Nature Methods) Monday April 6th 2015
Junjie Yao, Lidai Wang, Joon-Mo Yang, Konstantin I Maslov, Terence T W Wong, Lei Li, Chih-Hsien Huang, Jun Zou, Lihong V Wang

72) Identification of active transcriptional regulatory elements from GRO-seq data (Nature Methods) Monday March 23rd 2015
Charles G Danko, Stephanie L Hyland, Leighton J Core, Andre L Martins, Colin T Waters, Hyung Won Lee, Vivian G Cheung, W Lee Kraus, John T Lis, Adam Siepel

73) Improved specificity of TALE-based genome editing using an expanded RVD repertoire (Nature Methods) Monday March 23rd 2015
Jeffrey C Miller, Lei Zhang, Danny F Xia, John J Campo, Irina V Ankoudinova, Dmitry Y Guschin, Joshua E Babiarz, Xiangdong Meng, Sarah J Hinkley, Stephen C Lam, David E Paschon, Anna I Vincent, Gladys P Dulay, Kyle A Barlow, David A Shivak, Elo Leung, Jinwon D Kim, Rainier Amora, Fyodor D Urnov, Philip D Gregory, Edward J Rebar

74) Sensitive detection of chromatin-altering polymorphisms reveals autoimmune disease mechanisms (Nature Methods) Monday March 23rd 2015
Ricardo Cruz-Herrera del Rosario, Jeremie Poschmann, Sigrid Laure Rouam, Eileen Png, Chiea Chuen Khor, Martin Lloyd Hibberd, Shyam Prabhakar

75) Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes (Nature Methods) Monday March 23rd 2015
Anja Hasenberg, Mike Hasenberg, Linda M, Franziska Neumann, Lars Borkenstein, Manuel Stecher, Andreas Kraus, Daniel R Engel, Anika Klingberg, Pegah Seddigh, Zeinab Abdullah, Sabrina Klebow, Swen Engelmann, Annegret Reinhold, Sven Brandau, Michaela Seeling, Ari Waisman, Burkhart Schraven, Joachim R G, Falk Nimmerjahn, Matthias Gunzer

76) HISAT: a fast spliced aligner with low memory requirements (Nature Methods) Monday March 23rd 2015
Daehwan Kim, Ben Langmead, Steven L Salzberg

77) Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy (Nature Methods) Monday March 23rd 2015
Philip J Santangelo, Kenneth A Rogers, Chiara Zurla, Emeline L Blanchard, Sanjeev Gumber, Karen Strait, Fawn Connor-Stroud, David M Schuster, Praveen K Amancha, Jung Joo Hong, Siddappa N Byrareddy, James A Hoxie, Brani Vidakovic, Aftab A Ansari, Eric Hunter, Francois Villinger

78) Functional annotation of native enhancers with a Cas9 (Nature Methods) Monday March 23rd 2015
Nicola A Kearns, Hannah Pham, Barbara Tabak, Ryan M Genga, Noah J Silverstein, Manuel Garber, Ren

79) CIDRE: an illumination-correction method for optical microscopy (Nature Methods) Monday March 23rd 2015
Kevin Smith, Yunpeng Li, Filippo Piccinini, Gabor Csucs, Csaba Balazs, Alessandro Bevilacqua, Peter Horvath

80) Direct visualization of newly synthesized target proteins in situ (Nature Methods) Monday March 23rd 2015
Susanne tom Dieck, Lisa Kochen, Cyril Hanus, Maximilian Heum, Ina Bartnik, Belquis Nassim-Assir, Katrin Merk, Thorsten Mosler, Sakshi Garg, Stefanie Bunse, David A Tirrell, Erin M Schuman

81) Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing (Nature Methods) Monday March 23rd 2015
Michael B Clark, Tim R Mercer, Giovanni Bussotti, Tommaso Leonardi, Katelin R Haynes, Joanna Crawford, Marion E Brunck, Kim-Anh L, Gethin P Thomas, Wendy Y Chen, Ryan J Taft, Lars K Nielsen, Anton J Enright, John S Mattick, Marcel E Dinger

82) Highly efficient Cas9-mediated transcriptional programming (Nature Methods) Monday March 23rd 2015
Alejandro Chavez, Jonathan Scheiman, Suhani Vora, Benjamin W Pruitt, Marcelle Tuttle, Eswar P R Iyer, Shuailiang Lin, Samira Kiani, Christopher D Guzman, Daniel J Wiegand, Dmitry Ter-Ovanesyan, Jonathan L Braff, Noah Davidsohn, Benjamin E Housden, Norbert Perrimon, Ron Weiss, John Aach, James J Collins, George M Church

83) High-throughput RNA profiling via up-front sample parallelization (Nature Methods) Monday March 23rd 2015
Azeet Narayan, Ananth Bommakanti, Abhijit A Patel

84) Simultaneous generation of many RNA-seq libraries in a single reaction (Nature Methods) Monday March 23rd 2015
Alexander A Shishkin, Georgia Giannoukos, Alper Kucukural, Dawn Ciulla, Michele Busby, Christine Surka, Jenny Chen, Roby P Bhattacharyya, Robert F Rudy, Milesh M Patel, Nathaniel Novod, Deborah T Hung, Andreas Gnirke, Manuel Garber, Mitchell Guttman, Jonathan Livny

85) Genome sequence (Nature Methods) Monday March 23rd 2015
Qing Zhang, Xinshu Xiao

86) Atomic-accuracy models from 4.5- (Nature Methods) Monday February 23rd 2015
Frank DiMaio, Yifan Song, Xueming Li, Matthias J Brunner, Chunfu Xu, Vincent Conticello, Edward Egelman, Thomas C Marlovits, Yifan Cheng, David Baker

87) Image fusion of mass spectrometry and microscopy: a multimodality paradigm for molecular tissue mapping (Nature Methods) Monday February 23rd 2015
Raf Van de Plas, Junhai Yang, Jeffrey Spraggins, Richard M Caprioli

88) Improved data analysis for the MinION nanopore sequencer (Nature Methods) Monday February 23rd 2015
Miten Jain, Ian T Fiddes, Karen H Miga, Hugh E Olsen, Benedict Paten, Mark Akeson

89) De novo protein structure determination from near-atomic-resolution cryo-EM maps (Nature Methods) Monday February 23rd 2015
Ray Yu-Ruei Wang, Mikhail Kudryashev, Xueming Li, Edward H Egelman, Marek Basler, Yifan Cheng, David Baker, Frank DiMaio

90) Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics (Nature Methods) Monday February 23rd 2015
Kenneth J Hayworth, C Shan Xu, Zhiyuan Lu, Graham W Knott, Richard D Fetter, Juan Carlos Tapia, Jeff W Lichtman, Harald F Hess

91) Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells (Nature Methods) Monday February 9th 2015
Daesik Kim, Sangsu Bae, Jeongbin Park, Eunji Kim, Seokjoong Kim, Hye Ryeong Yu, Jinha Hwang, Jong-Il Kim, Jin-Soo Kim

92) Mapping native disulfide bonds at a proteome scale (Nature Methods) Monday February 9th 2015
Shan Lu, Sheng-Bo Fan, Bing Yang, Yu-Xin Li, Jia-Ming Meng, Long Wu, Pin Li, Kun Zhang, Mei-Jun Zhang, Yan Fu, Jincai Luo, Rui-Xiang Sun, Si-Min He, Meng-Qiu Dong

93) Accurate liability estimation improves power in ascertained case-control studies (Nature Methods) Monday February 9th 2015
Omer Weissbrod, Christoph Lippert, Dan Geiger, David Heckerman

94) A sensor for quantification of macromolecular crowding in living cells (Nature Methods) Monday February 9th 2015
Arnold J Boersma, Inge S Zuhorn, Bert Poolman

95) Reporters for sensitive and quantitative measurement of auxin response (Nature Methods) Monday February 9th 2015
Che-Yang Liao, Wouter Smet, Geraldine Brunoud, Saiko Yoshida, Teva Vernoux, Dolf Weijers

96) Real-time deformability cytometry: on-the-fly cell mechanical phenotyping (Nature Methods) Monday February 9th 2015
Oliver Otto, Philipp Rosendahl, Alexander Mietke, Stefan Golfier, Christoph Herold, Daniel Klaue, Salvatore Girardo, Stefano Pagliara, Andrew Ekpenyong, Angela Jacobi, Manja Wobus, Nicole T, Ulrich F Keyser, J, Elisabeth Fischer-Friedrich, Jochen Guck

97) Ribose-seq: global mapping of ribonucleotides embedded in genomic DNA (Nature Methods) Monday January 26th 2015
Kyung Duk Koh, Sathya Balachander, Jay R Hesselberth, Francesca Storici

98) A general method to improve fluorophores for live-cell and single-molecule microscopy (Nature Methods) Monday January 26th 2015
Jonathan B Grimm, Brian P English, Jiji Chen, Joel P Slaughter, Zhengjian Zhang, Andrey Revyakin, Ronak Patel, John J Macklin, Davide Normanno, Robert H Singer, Timoth, Luke D Lavis

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Mitch Andre Garcia's Chem Feeds 2008-present

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