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1) In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy (Nature Methods) Monday April 13th 2015
Deepak R Chittajallu, Stefan Florian, Rainer H Kohler, Yoshiko Iwamoto, James D Orth, Ralph Weissleder, Gaudenz Danuser, Timothy J Mitchison

2) High-resolution whole-brain staining for electron microscopic circuit reconstruction (Nature Methods) Monday April 13th 2015
Shawn Mikula, Winfried Denk

3) Rapid reverse genetic screening using CRISPR in zebrafish (Nature Methods) Monday April 13th 2015
Arish N Shah, Crystal F Davey, Alex C Whitebirch, Adam C Miller, Cecilia B Moens

4) Massively parallel delivery of large cargo into mammalian cells with light pulses (Nature Methods) Monday April 6th 2015
Yi-Chien Wu, Ting-Hsiang Wu, Daniel L Clemens, Bai-Yu Lee, Ximiao Wen, Marcus A Horwitz, Michael A Teitell, Pei-Yu Chiou

5) Robust enumeration of cell subsets from tissue expression profiles (Nature Methods) Monday April 6th 2015
Aaron M Newman, Chih Long Liu, Michael R Green, Andrew J Gentles, Weiguo Feng, Yue Xu, Chuong D Hoang, Maximilian Diehn, Ash A Alizadeh

6) Quantifying cellular capacity identifies gene expression designs with reduced burden (Nature Methods) Monday April 6th 2015
Francesca Ceroni, Rhys Algar, Guy-Bart Stan, Tom Ellis

7) Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra (Nature Methods) Monday April 6th 2015
Daniel Franke, Cy M Jeffries, Dmitri I Svergun

8) Sequencing small genomic targets with high efficiency and extreme accuracy (Nature Methods) Monday April 6th 2015
Michael W Schmitt, Edward J Fox, Marc J Prindle, Kate S Reid-Bayliss, Lawrence D True, Jerald P Radich, Lawrence A Loeb

9) High-speed label-free functional photoacoustic microscopy of mouse brain in action (Nature Methods) Monday April 6th 2015
Junjie Yao, Lidai Wang, Joon-Mo Yang, Konstantin I Maslov, Terence T W Wong, Lei Li, Chih-Hsien Huang, Jun Zou, Lihong V Wang

10) Identification of active transcriptional regulatory elements from GRO-seq data (Nature Methods) Monday March 23rd 2015
Charles G Danko, Stephanie L Hyland, Leighton J Core, Andre L Martins, Colin T Waters, Hyung Won Lee, Vivian G Cheung, W Lee Kraus, John T Lis, Adam Siepel

11) Improved specificity of TALE-based genome editing using an expanded RVD repertoire (Nature Methods) Monday March 23rd 2015
Jeffrey C Miller, Lei Zhang, Danny F Xia, John J Campo, Irina V Ankoudinova, Dmitry Y Guschin, Joshua E Babiarz, Xiangdong Meng, Sarah J Hinkley, Stephen C Lam, David E Paschon, Anna I Vincent, Gladys P Dulay, Kyle A Barlow, David A Shivak, Elo Leung, Jinwon D Kim, Rainier Amora, Fyodor D Urnov, Philip D Gregory, Edward J Rebar

12) Sensitive detection of chromatin-altering polymorphisms reveals autoimmune disease mechanisms (Nature Methods) Monday March 23rd 2015
Ricardo Cruz-Herrera del Rosario, Jeremie Poschmann, Sigrid Laure Rouam, Eileen Png, Chiea Chuen Khor, Martin Lloyd Hibberd, Shyam Prabhakar

13) Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes (Nature Methods) Monday March 23rd 2015
Anja Hasenberg, Mike Hasenberg, Linda M, Franziska Neumann, Lars Borkenstein, Manuel Stecher, Andreas Kraus, Daniel R Engel, Anika Klingberg, Pegah Seddigh, Zeinab Abdullah, Sabrina Klebow, Swen Engelmann, Annegret Reinhold, Sven Brandau, Michaela Seeling, Ari Waisman, Burkhart Schraven, Joachim R G, Falk Nimmerjahn, Matthias Gunzer

14) HISAT: a fast spliced aligner with low memory requirements (Nature Methods) Monday March 23rd 2015
Daehwan Kim, Ben Langmead, Steven L Salzberg

15) Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy (Nature Methods) Monday March 23rd 2015
Philip J Santangelo, Kenneth A Rogers, Chiara Zurla, Emeline L Blanchard, Sanjeev Gumber, Karen Strait, Fawn Connor-Stroud, David M Schuster, Praveen K Amancha, Jung Joo Hong, Siddappa N Byrareddy, James A Hoxie, Brani Vidakovic, Aftab A Ansari, Eric Hunter, Francois Villinger

16) Functional annotation of native enhancers with a Cas9 (Nature Methods) Monday March 23rd 2015
Nicola A Kearns, Hannah Pham, Barbara Tabak, Ryan M Genga, Noah J Silverstein, Manuel Garber, Ren

17) CIDRE: an illumination-correction method for optical microscopy (Nature Methods) Monday March 23rd 2015
Kevin Smith, Yunpeng Li, Filippo Piccinini, Gabor Csucs, Csaba Balazs, Alessandro Bevilacqua, Peter Horvath

18) Direct visualization of newly synthesized target proteins in situ (Nature Methods) Monday March 23rd 2015
Susanne tom Dieck, Lisa Kochen, Cyril Hanus, Maximilian Heum, Ina Bartnik, Belquis Nassim-Assir, Katrin Merk, Thorsten Mosler, Sakshi Garg, Stefanie Bunse, David A Tirrell, Erin M Schuman

19) Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing (Nature Methods) Monday March 23rd 2015
Michael B Clark, Tim R Mercer, Giovanni Bussotti, Tommaso Leonardi, Katelin R Haynes, Joanna Crawford, Marion E Brunck, Kim-Anh L, Gethin P Thomas, Wendy Y Chen, Ryan J Taft, Lars K Nielsen, Anton J Enright, John S Mattick, Marcel E Dinger

20) Highly efficient Cas9-mediated transcriptional programming (Nature Methods) Monday March 23rd 2015
Alejandro Chavez, Jonathan Scheiman, Suhani Vora, Benjamin W Pruitt, Marcelle Tuttle, Eswar P R Iyer, Shuailiang Lin, Samira Kiani, Christopher D Guzman, Daniel J Wiegand, Dmitry Ter-Ovanesyan, Jonathan L Braff, Noah Davidsohn, Benjamin E Housden, Norbert Perrimon, Ron Weiss, John Aach, James J Collins, George M Church

21) High-throughput RNA profiling via up-front sample parallelization (Nature Methods) Monday March 23rd 2015
Azeet Narayan, Ananth Bommakanti, Abhijit A Patel

22) Simultaneous generation of many RNA-seq libraries in a single reaction (Nature Methods) Monday March 23rd 2015
Alexander A Shishkin, Georgia Giannoukos, Alper Kucukural, Dawn Ciulla, Michele Busby, Christine Surka, Jenny Chen, Roby P Bhattacharyya, Robert F Rudy, Milesh M Patel, Nathaniel Novod, Deborah T Hung, Andreas Gnirke, Manuel Garber, Mitchell Guttman, Jonathan Livny

23) Genome sequence (Nature Methods) Monday March 23rd 2015
Qing Zhang, Xinshu Xiao

24) Atomic-accuracy models from 4.5- (Nature Methods) Monday February 23rd 2015
Frank DiMaio, Yifan Song, Xueming Li, Matthias J Brunner, Chunfu Xu, Vincent Conticello, Edward Egelman, Thomas C Marlovits, Yifan Cheng, David Baker

25) Image fusion of mass spectrometry and microscopy: a multimodality paradigm for molecular tissue mapping (Nature Methods) Monday February 23rd 2015
Raf Van de Plas, Junhai Yang, Jeffrey Spraggins, Richard M Caprioli

26) Improved data analysis for the MinION nanopore sequencer (Nature Methods) Monday February 23rd 2015
Miten Jain, Ian T Fiddes, Karen H Miga, Hugh E Olsen, Benedict Paten, Mark Akeson

27) De novo protein structure determination from near-atomic-resolution cryo-EM maps (Nature Methods) Monday February 23rd 2015
Ray Yu-Ruei Wang, Mikhail Kudryashev, Xueming Li, Edward H Egelman, Marek Basler, Yifan Cheng, David Baker, Frank DiMaio

28) Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics (Nature Methods) Monday February 23rd 2015
Kenneth J Hayworth, C Shan Xu, Zhiyuan Lu, Graham W Knott, Richard D Fetter, Juan Carlos Tapia, Jeff W Lichtman, Harald F Hess

29) Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells (Nature Methods) Monday February 9th 2015
Daesik Kim, Sangsu Bae, Jeongbin Park, Eunji Kim, Seokjoong Kim, Hye Ryeong Yu, Jinha Hwang, Jong-Il Kim, Jin-Soo Kim

30) Mapping native disulfide bonds at a proteome scale (Nature Methods) Monday February 9th 2015
Shan Lu, Sheng-Bo Fan, Bing Yang, Yu-Xin Li, Jia-Ming Meng, Long Wu, Pin Li, Kun Zhang, Mei-Jun Zhang, Yan Fu, Jincai Luo, Rui-Xiang Sun, Si-Min He, Meng-Qiu Dong

31) Accurate liability estimation improves power in ascertained case-control studies (Nature Methods) Monday February 9th 2015
Omer Weissbrod, Christoph Lippert, Dan Geiger, David Heckerman

32) A sensor for quantification of macromolecular crowding in living cells (Nature Methods) Monday February 9th 2015
Arnold J Boersma, Inge S Zuhorn, Bert Poolman

33) Reporters for sensitive and quantitative measurement of auxin response (Nature Methods) Monday February 9th 2015
Che-Yang Liao, Wouter Smet, Geraldine Brunoud, Saiko Yoshida, Teva Vernoux, Dolf Weijers

34) Real-time deformability cytometry: on-the-fly cell mechanical phenotyping (Nature Methods) Monday February 9th 2015
Oliver Otto, Philipp Rosendahl, Alexander Mietke, Stefan Golfier, Christoph Herold, Daniel Klaue, Salvatore Girardo, Stefano Pagliara, Andrew Ekpenyong, Angela Jacobi, Manja Wobus, Nicole T, Ulrich F Keyser, J, Elisabeth Fischer-Friedrich, Jochen Guck

35) Ribose-seq: global mapping of ribonucleotides embedded in genomic DNA (Nature Methods) Monday January 26th 2015
Kyung Duk Koh, Sathya Balachander, Jay R Hesselberth, Francesca Storici

36) A general method to improve fluorophores for live-cell and single-molecule microscopy (Nature Methods) Monday January 26th 2015
Jonathan B Grimm, Brian P English, Jiji Chen, Joel P Slaughter, Zhengjian Zhang, Andrey Revyakin, Ronak Patel, John J Macklin, Davide Normanno, Robert H Singer, Timoth, Luke D Lavis

37) DIA-Umpire: comprehensive computational framework for data-independent acquisition proteomics (Nature Methods) Monday January 26th 2015
Chih-Chiang Tsou, Dmitry Avtonomov, Brett Larsen, Monika Tucholska, Hyungwon Choi, Anne-Claude Gingras, Alexey I Nesvizhskii

38) Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivo (Nature Methods) Monday January 26th 2015
Adam M Packer, Lloyd E Russell, Henry W P Dalgleish, Michael H

39) Selecting causal genes from genome-wide association studies via functionally coherent subnetworks (Nature Methods) Monday January 26th 2015
Murat Ta, Gabriel Musso, Tong Hao, Marc Vidal, Calum A MacRae, Frederick P Roth

40) Quantitative profiling of initiating ribosomes in vivo (Nature Methods) Monday January 26th 2015
Xiangwei Gao, Ji Wan, Botao Liu, Ming Ma, Ben Shen, Shu-Bing Qian

41) Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange (Nature Methods) Monday January 26th 2015
Yidan Ding, Jiao Li, Jhon Ralph Enterina, Yi Shen, Issan Zhang, Paul H Tewson, Gary C H Mo, Jin Zhang, Anne Marie Quinn, Thomas E Hughes, Dusica Maysinger, Spencer C Alford, Yan Zhang, Robert E Campbell

42) Inferring biological tasks using Pareto analysis of high-dimensional data (Nature Methods) Monday January 26th 2015
Yuval Hart, Hila Sheftel, Jean Hausser, Pablo Szekely, Noa Bossel Ben-Moshe, Yael Korem, Avichai Tendler, Avraham E Mayo, Uri Alon

43) Sequence tag (Nature Methods) Monday January 26th 2015
S, Pia Abel zur Wiesch, Hsiao-Han Chang, Brigid M Davis, Marc Lipsitch, Matthew K Waldor

44) Fixation-resistant photoactivatable fluorescent proteins for CLEM (Nature Methods) Monday January 26th 2015
Maria G Paez-Segala, Mei G Sun, Gleb Shtengel, Sarada Viswanathan, Michelle A Baird, John J Macklin, Ronak Patel, John R Allen, Elizabeth S Howe, Grzegorz Piszczek, Harald F Hess, Michael W Davidson, Yalin Wang, Loren L Looger

45) Improved and expanded Q-system reagents for genetic manipulations (Nature Methods) Monday January 26th 2015
Olena Riabinina, David Luginbuhl, Elizabeth Marr, Sha Liu, Mark N Wu, Liqun Luo, Christopher J Potter

46) Targeted exploration and analysis of large cross-platform human transcriptomic compendia (Nature Methods) Monday January 26th 2015
Qian Zhu, Aaron K Wong, Arjun Krishnan, Miriam R Aure, Alicja Tadych, Ran Zhang, David C Corney, Casey S Greene, Lars A Bongo, Vessela N Kristensen, Moses Charikar, Kai Li, Olga G Troyanskaya

47) Massively parallel single-amino-acid mutagenesis (Nature Methods) Monday January 26th 2015
Jacob O Kitzman, Lea M Starita, Russell S Lo, Stanley Fields, Jay Shendure

48) Macromolecular X-ray structure determination using weak, single-wavelength anomalous data (Nature Methods) Monday January 26th 2015
G, Airlie J McCoy, Nathaniel Echols, Ralf W Grosse-Kunstleve, Paul D Adams, James M Holton, Randy J Read, Thomas C Terwilliger

49) Conformal nanopatterning of extracellular matrix proteins onto topographically complex surfaces (Nature Methods) Monday January 26th 2015
Yan Sun, Quentin Jallerat, John M Szymanski, Adam W Feinberg

50) Fast native-SAD phasing for routine macromolecular structure determination (Nature Methods) Monday January 26th 2015
Tobias Weinert, Vincent Olieric, Sandro Waltersperger, Ezequiel Panepucci, Lirong Chen, Hua Zhang, Dayong Zhou, John Rose, Akio Ebihara, Seiki Kuramitsu, Dianfan Li, Nicole Howe, Gisela Schnapp, Alexander Pautsch, Katja Bargsten, Andrea E Prota, Parag Surana, Jithesh Kottur, Deepak T Nair, Federica Basilico, Valentina Cecatiello, Sebastiano Pasqualato, Andreas Boland, Oliver Weichenrieder, Bi-Cheng Wang, Michel O Steinmetz, Martin Caffrey, Meitian Wang

51) DNA nanoswitches: a quantitative platform for gel-based biomolecular interaction analysis (Nature Methods) Monday January 26th 2015
Mounir A Koussa, Ken Halvorsen, Andrew Ward, Wesley P Wong

52) Genetic targeting of chemical indicators in vivo (Nature Methods) Monday January 26th 2015
Guoying Yang, Fernanda de Castro Reis, Mayya Sundukova, Sofia Pimpinella, Antonino Asaro, Laura Castaldi, Laura Batti, Daniel Bilbao, Luc Reymond, Kai Johnsson, Paul A Heppenstall

53) Grease matrix as a versatile carrier of proteins for serial crystallography (Nature Methods) Monday November 10th 2014
Michihiro Sugahara, Eiichi Mizohata, Eriko Nango, Mamoru Suzuki, Tomoyuki Tanaka, Tetsuya Masuda, Rie Tanaka, Tatsuro Shimamura, Yoshiki Tanaka, Chiyo Suno, Kentaro Ihara, Dongqing Pan, Keisuke Kakinouchi, Shigeru Sugiyama, Michio Murata, Tsuyoshi Inoue, Kensuke Tono, Changyong Song, Jaehyun Park, Takashi Kameshima, Takaki Hatsui, Yasumasa Joti, Makina Yabashi, So Iwata

54) A robust pipeline for rapid production of versatile nanobody repertoires (Nature Methods) Sunday November 2nd 2014
Peter C Fridy, Yinyin Li, Sarah Keegan, Mary K Thompson, Ilona Nudelman, Johannes F Scheid, Marlene Oeffinger, Michel C Nussenzweig, David Feny, Brian T Chait, Michael P Rout

55) Rovers minimize human disturbance in research on wild animals (Nature Methods) Sunday November 2nd 2014
Yvon Le Maho, Jason D Whittington, Nicolas Hanuise, Louise Pereira, Matthieu Boureau, Mathieu Brucker, Nicolas Chatelain, Julien Courtecuisse, Francis Crenner, Benjamin Friess, Edith Grosbellet, La, Fr, Claire Saraux, Nathana, Vincent A Viblanc, Bernard Thierry, Pascale Tremblay, Ren, C

56) Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing (Nature Methods) Sunday November 2nd 2014
Michael J Ziller, Kasper D Hansen, Alexander Meissner, Martin J Aryee

57) Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex (Nature Methods) Sunday October 26th 2014
Arnaud Muller, Victory Joseph, Paul A Slesinger, David Kleinfeld

58) Permanent genetic memory with gt;1-byte capacity (Nature Methods) Sunday October 26th 2014
Lei Yang, Alec A K Nielsen, Jesus Fernandez-Rodriguez, Conor J McClune, Michael T Laub, Timothy K Lu, Christopher A Voigt

59) Quantum dot (Nature Methods) Sunday October 26th 2014
Bertalan K Andr, Gregorio L Gali, Daniel Huber, Mladen Barbic, John J Macklin, Kimihiro Susumu, James B Delehanty, Alan L Huston, Judit K Makara, Igor L Medintz

60) A general design strategy for protein-responsive riboswitches in mammalian cells (Nature Methods) Sunday October 12th 2014
Simon Ausl, Pascal St, Charlotte Rehm, David Ausl, J, Martin Fussenegger

61) Intracellular autofluorescence: a biomarker for epithelial cancer stem cells (Nature Methods) Sunday October 12th 2014
Irene Miranda-Lorenzo, Jorge Dorado, Enza Lonardo, Sonia Alcala, Alicia G Serrano, Jenifer Clausell-Tormos, Michele Cioffi, Diego Megias, Sladjana Zagorac, Anamaria Balic, Manuel Hidalgo, Mert Erkan, Joerg Kleeff, Aldo Scarpa, Bruno Sainz, Christopher Heeschen

62) Small molecules facilitate rapid and synchronous iPSC generation (Nature Methods) Sunday October 12th 2014
Ori Bar-Nur, Justin Brumbaugh, Cassandra Verheul, Effie Apostolou, Iulian Pruteanu-Malinici, Ryan M Walsh, Sridhar Ramaswamy, Konrad Hochedlinger

63) Predicting the human epigenome from DNA motifs (Nature Methods) Sunday October 12th 2014
John W Whitaker, Zhao Chen, Wei Wang

64) A quantitative framework for the forward design of synthetic miRNA circuits (Nature Methods) Sunday October 12th 2014
Ryan J Bloom, Sally M Winkler, Christina D Smolke

65) Nanoparticle vesicle encoding for imaging and tracking cell populations (Nature Methods) Sunday October 12th 2014
Paul Rees, John W Wills, M Rowan Brown, James Tonkin, Mark D Holton, Nicole Hondow, Andrew P Brown, Rik Brydson, Val Millar, Anne E Carpenter, Huw D Summers

66) A DNA-based molecular probe for optically reporting cellular traction forces (Nature Methods) Sunday October 12th 2014
Brandon L Blakely, Christoph E Dumelin, Britta Trappmann, Lynn M McGregor, Colin K Choi, Peter C Anthony, Van K Duesterberg, Brendon M Baker, Steven M Block, David R Liu, Christopher S Chen

67) An improved surface passivation method for single-molecule studies (Nature Methods) Sunday October 12th 2014
Boyang Hua, Kyu Young Han, Ruobo Zhou, Hajin Kim, Xinghua Shi, Sanjaya C Abeysirigunawardena, Ankur Jain, Digvijay Singh, Vasudha Aggarwal, Sarah A Woodson, Taekjip Ha

68) Time-resolved crystallography using the Hadamard transform (Nature Methods) Sunday October 12th 2014
Briony A Yorke, Godfrey S Beddard, Robin L Owen, Arwen R Pearson

69) Reconstruction of vocal interactions in a group of small songbirds (Nature Methods) Sunday October 12th 2014
Victor N Anisimov, Joshua A Herbst, Andrei N Abramchuk, Alexander V Latanov, Richard H R Hahnloser, Alexei L Vyssotski

70) Comprehensive analysis of DNA methylation data with RnBeads (Nature Methods) Sunday October 12th 2014
Yassen Assenov, Fabian M, Pavlo Lutsik, J, Thomas Lengauer, Christoph Bock

71) 3D genome reconstruction from chromosomal contacts (Nature Methods) Sunday October 12th 2014
Annick Lesne, Julien Riposo, Paul Roger, Axel Cournac, Julien Mozziconacci

72) Binning metagenomic contigs by coverage and composition (Nature Methods) Sunday October 12th 2014
Johannes Alneberg, Brynjar Sm, Ino de Bruijn, Melanie Schirmer, Joshua Quick, Umer Z Ijaz, Leo Lahti, Nicholas J Loman, Anders F Andersson, Christopher Quince

73) From genes to protein mechanics on a chip (Nature Methods) Sunday October 12th 2014
Marcus Otten, Wolfgang Ott, Markus A Jobst, Lukas F Milles, Tobias Verdorfer, Diana A Pippig, Michael A Nash, Hermann E Gaub

74) Light-sheet functional imaging in fictively behaving zebrafish (Nature Methods) Sunday August 17th 2014
Nikita Vladimirov, Yu Mu, Takashi Kawashima, Davis V Bennett, Chao-Tsung Yang, Loren L Looger, Philipp J Keller, Jeremy Freeman, Misha B Ahrens

75) Mapping brain activity at scale with cluster computing (Nature Methods) Sunday August 17th 2014
Jeremy Freeman, Nikita Vladimirov, Takashi Kawashima, Yu Mu, Nicholas J Sofroniew, Davis V Bennett, Joshua Rosen, Chao-Tsung Yang, Loren L Looger, Misha B Ahrens

76) Multiplexed aberration measurement for deep tissue imaging in vivo (Nature Methods) Sunday August 17th 2014
Chen Wang, Rui Liu, Daniel E Milkie, Wenzhi Sun, Zhongchao Tan, Aaron Kerlin, Tsai-Wen Chen, Douglas S Kim, Na Ji

77) Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser (Nature Methods) Sunday August 17th 2014
David Arnlund, Linda C Johansson, Cecilia Wickstrand, Anton Barty, Garth J Williams, Erik Malmerberg, Jan Davidsson, Despina Milathianaki, Daniel P DePonte, Robert L Shoeman, Dingjie Wang, Daniel James, Gergely Katona, Sebastian Westenhoff, Thomas A White, Andrew Aquila, Sadia Bari, Peter Berntsen, Mike Bogan, Tim Brandt van Driel, R Bruce Doak, Kasper Skov Kj, Matthias Frank, Raimund Fromme, Ingo Grotjohann, Robert Henning, Mark S Hunter, Richard A Kirian, Irina Kosheleva, Christopher Kupitz, Mengning Liang, Andrew V Martin, Martin Meedom Nielsen, Marc Messerschmidt, M Marvin Seibert, Jennie Sj, Francesco Stellato, Uwe Weierstall, Nadia A Zatsepin, John C H Spence, Petra Fromme, Ilme Schlichting, S, Gerrit Groenhof, Henry N Chapman, Richard Neutze

78) Efficient CRISPR-Cas9 (Nature Methods) Sunday August 17th 2014
Jeffrey C Wagner, Randall J Platt, Stephen J Goldfless, Feng Zhang, Jacquin C Niles

79) Epiviz: interactive visual analytics for functional genomics data (Nature Methods) Sunday August 17th 2014
Florin Chelaru, Llewellyn Smith, Naomi Goldstein, H

80) Phen-Gen: combining phenotype and genotype to analyze rare disorders (Nature Methods) Sunday August 17th 2014
Asif Javed, Saloni Agrawal, Pauline C Ng

81) Nanoscale high-content analysis using compositional heterogeneities of single proteoliposomes (Nature Methods) Sunday August 17th 2014
Signe Mathiasen, Sune M Christensen, Juan Jos, S, Jonathan F Fay, Sune K Jorgensen, Salome Veshaguri, David L Farrens, Maria Kiskowski, Brian Kobilka, Dimitrios Stamou

82) High-resolution structure determination by continuous-rotation data collection in MicroED (Nature Methods) Sunday August 17th 2014
Brent L Nannenga, Dan Shi, Andrew G W Leslie, Tamir Gonen

83) Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis (Nature Methods) Sunday July 20th 2014
George T Lyozin, Paul C Bressloff, Amit Kumar, Yasuhiro Kosaka, Bradley L Demarest, H Joseph Yost, Michael R Kuehn, Luca Brunelli

84) RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP) (Nature Methods) Sunday July 20th 2014
Nathan A Siegfried, Steven Busan, Greggory M Rice, Julie A E Nelson, Kevin M Weeks

85) Fast, accurate reconstruction of cell lineages from large-scale fluorescence microscopy data (Nature Methods) Sunday July 20th 2014
Fernando Amat, William Lemon, Daniel P Mossing, Katie McDole, Yinan Wan, Kristin Branson, Eugene W Myers, Philipp J Keller

86) High-resolution reconstruction of the beating zebrafish heart (Nature Methods) Sunday July 20th 2014
Michaela Mickoleit, Benjamin Schmid, Michael Weber, Florian O Fahrbach, Sonja Hombach, Sven Reischauer, Jan Huisken

87) Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity (Nature Methods) Sunday July 20th 2014
S, Heather J Lee, Christof Angermueller, Felix Krueger, Heba Saadeh, Julian Peat, Simon R Andrews, Oliver Stegle, Wolf Reik, Gavin Kelsey

88) Optimized cell transplantation using adult rag2 mutant zebrafish (Nature Methods) Sunday July 20th 2014
Qin Tang, Nouran S Abdelfattah, Jessica S Blackburn, John C Moore, Sarah A Martinez, Finola E Moore, Riadh Lobbardi, In, Myron S Ignatius, Jason N Berman, Robert S Liwski, Yariv Houvras, David M Langenau

89) Accelerated chromatin biochemistry using DNA-barcoded nucleosome libraries (Nature Methods) Sunday July 6th 2014
Uyen T T Nguyen, Lenka Bittova, Manuel M M, Beat Fierz, Yael David, Brian Houck-Loomis, Vanessa Feng, Geoffrey P Dann, Tom W Muir

90) Spatial control of membrane receptor function using ligand nanocalipers (Nature Methods) Sunday July 6th 2014
Alan Shaw, Vanessa Lundin, Ekaterina Petrova, Ferenc F, Erik Benson, Abdullah Al-Amin, Anna Herland, Andries Blokzijl, Bj, Ana I Teixeira

91) A method to recapitulate early embryonic spatial patterning in human embryonic stem cells (Nature Methods) Sunday June 29th 2014
Aryeh Warmflash, Benoit Sorre, Fred Etoc, Eric D Siggia, Ali H Brivanlou

92) All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins (Nature Methods) Sunday June 22nd 2014
Daniel R Hochbaum, Yongxin Zhao, Samouil L Farhi, Nathan Klapoetke, Christopher A Werley, Vikrant Kapoor, Peng Zou, Joel M Kralj, Dougal Maclaurin, Niklas Smedemark-Margulies, Jessica L Saulnier, Gabriella L Boulting, Christoph Straub, Yong Ku Cho, Michael Melkonian, Gane Ka-Shu Wong, D Jed Harrison, Venkatesh N Murthy, Bernardo L Sabatini, Edward S Boyden, Robert E Campbell, Adam E Cohen

93) Annotation of loci from genome-wide association studies using tissue-specific quantitative interaction proteomics (Nature Methods) Sunday June 22nd 2014
Alicia Lundby, Elizabeth J Rossin, Annette B Steffensen, Moshe Rav Acha, Christopher Newton-Cheh, Arne Pfeufer, Stacey N Lynch, S, S, Patrick T Ellinor, J Wouter Jukema, Stella Trompet, Ian Ford, Peter W Macfarlane, Bouwe P Krijthe, Albert Hofman, Andr, Bruno H Stricker, Hendrik M Nathoe, Wilko Spiering, Mark J Daly, Folkert W Asselbergs, Pim van der Harst, David J Milan, Paul I W de Bakker, Kasper Lage, Jesper V Olsen

94) Chemically defined generation of human cardiomyocytes (Nature Methods) Sunday June 15th 2014
Paul W Burridge, Elena Matsa, Praveen Shukla, Ziliang C Lin, Jared M Churko, Antje D Ebert, Feng Lan, Sebastian Diecke, Bruno Huber, Nicholas M Mordwinkin, Jordan R Plews, Oscar J Abilez, Bianxiao Cui, Joseph D Gold, Joseph C Wu

95) Protein delivery into live cells by incubation with an endosomolytic agent (Nature Methods) Sunday June 15th 2014
Alfredo Erazo-Oliveras, Kristina Najjar, Laila Dayani, Ting-Yi Wang, Gregory A Johnson, Jean-Philippe Pellois

96) Targeting cells with single vectors using multiple-feature Boolean logic (Nature Methods) Sunday June 8th 2014
Lief E Fenno, Joanna Mattis, Charu Ramakrishnan, Minsuk Hyun, Soo Yeun Lee, Miao He, Jason Tucciarone, Aslihan Selimbeyoglu, Andre Berndt, Logan Grosenick, Kelly A Zalocusky, Hannah Bernstein, Haley Swanson, Chelsey Perry, Ilka Diester, Frederick M Boyce, Caroline E Bass, Rachael Neve, Z Josh Huang, Karl Deisseroth

97) Single-cell western blotting (Nature Methods) Sunday June 1st 2014
Alex J Hughes, Dawn P Spelke, Zhuchen Xu, Chi-Chih Kang, David V Schaffer, Amy E Herr

98) idTracker: tracking individuals in a group by automatic identification of unmarked animals (Nature Methods) Sunday June 1st 2014
Alfonso P, Juli, Robert C Hinz, Sara Arganda, Gonzalo G de Polavieja

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