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1) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew (Nature Biotechnology) Sunday July 20th 2014
Yanpeng Wang, Xi Cheng, Qiwei Shan, Yi Zhang, Jinxing Liu, Caixia Gao, Jin-Long Qiu

2) Identification and assembly of genomes and genetic elements in complex metagenomic samples without using reference genomes (Nature Biotechnology) Sunday July 6th 2014
H Bj, Mathieu Almeida, Agnieszka Sierakowska Juncker, Simon Rasmussen, Junhua Li, Shinichi Sunagawa, Damian R Plichta, Laurent Gautier, Anders G Pedersen, Emmanuelle Le Chatelier, Eric Pelletier, Ida Bonde, Trine Nielsen, Chaysavanh Manichanh, Manimozhiyan Arumugam, Jean-Michel Batto, Marcelo B Quintanilha dos Santos, Nikolaj Blom, Natalia Borruel, Kristoffer S Burgdorf, Fouad Boumezbeur, Francesc Casellas, Jo, Piotr Dworzynski, Francisco Guarner, Torben Hansen, Falk Hildebrand, Rolf S Kaas, Sean Kennedy, Karsten Kristiansen, Jens Roat Kultima, Pierre L, Florence Levenez, Ole Lund, Bouziane Moumen, Denis Le Paslier, Nicolas Pons, Oluf Pedersen, Edi Prifti, Junjie Qin, Jeroen Raes, S, Julien Tap, Sebastian Tims, David W Ussery, Takuji Yamada, Pierre Renault, Thomas Sicheritz-Ponten, Peer Bork, Jun Wang, S, S Dusko Ehrlich

3) Revealing long noncoding RNA architecture and functions using domain-specific chromatin isolation by RNA purification (Nature Biotechnology) Sunday July 6th 2014
Jeffrey J Quinn, Ibrahim A Ilik, Kun Qu, Plamen Georgiev, Ci Chu, Asifa Akhtar, Howard Y Chang

4) An integrated catalog of reference genes in the human gut microbiome (Nature Biotechnology) Sunday July 6th 2014
Junhua Li, Huijue Jia, Xianghang Cai, Huanzi Zhong, Qiang Feng, Shinichi Sunagawa, Manimozhiyan Arumugam, Jens Roat Kultima, Edi Prifti, Trine Nielsen, Agnieszka Sierakowska Juncker, Chaysavanh Manichanh, Bing Chen, Wenwei Zhang, Florence Levenez, Juan Wang, Xun Xu, Liang Xiao, Suisha Liang, Dongya Zhang, Zhaoxi Zhang, Weineng Chen, Hailong Zhao, Jumana Yousuf Al-Aama, Sherif Edris, Huanming Yang, Jian Wang, Torben Hansen, Henrik Bj, S, Karsten Kristiansen, Francisco Guarner, Oluf Pedersen, Joel Dor, S Dusko Ehrlich, Peer Bork, Jun Wang

5) Decoding long nanopore sequencing reads of natural DNA (Nature Biotechnology) Wednesday June 25th 2014
Andrew H Laszlo, Ian M Derrington, Brian C Ross, Henry Brinkerhoff, Andrew Adey, Ian C Nova, Jonathan M Craig, Kyle W Langford, Jenny Mae Samson, Riza Daza, Kenji Doering, Jay Shendure, Jens H Gundlach

6) Linking T-cell receptor sequence to functional phenotype at the single-cell level (Nature Biotechnology) Sunday June 22nd 2014
Arnold Han, Jacob Glanville, Leo Hansmann, Mark M Davis

7) Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing (Nature Biotechnology) Sunday June 22nd 2014
Dirk Heckl, Monika S Kowalczyk, David Yudovich, Roger Belizaire, Rishi V Puram, Marie E McConkey, Anne Thielke, Jon C Aster, Aviv Regev, Benjamin L Ebert

8) Assessing the clinical utility of cancer genomic and proteomic data across tumor types (Nature Biotechnology) Sunday June 22nd 2014
Yuan Yuan, Eliezer M Van Allen, Larsson Omberg, Nikhil Wagle, Ali Amin-Mansour, Artem Sokolov, Lauren A Byers, Yanxun Xu, Kenneth R Hess, Lixia Diao, Leng Han, Xuelin Huang, Michael S Lawrence, John N Weinstein, Josh M Stuart, Gordon B Mills, Levi A Garraway, Adam A Margolin, Gad Getz, Han Liang

9) Backbone modification of a polypeptide drug alters duration of action in vivo (Nature Biotechnology) Sunday June 15th 2014
Ross W Cheloha, Akira Maeda, Thomas Dean, Thomas J Gardella, Samuel H Gellman

10) Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system (Nature Biotechnology) Sunday June 15th 2014
Mehdi Ghorbal, Molly Gorman, Cameron Ross Macpherson, Rafael Miyazawa Martins, Artur Scherf, Jose-Juan Lopez-Rubio

11) Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication (Nature Biotechnology) Sunday June 15th 2014
G Albert Wu, Simon Prochnik, Jerry Jenkins, Jerome Salse, Uffe Hellsten, Florent Murat, Xavier Perrier, Manuel Ruiz, Simone Scalabrin, Javier Terol, Marco Aur, Karine Labadie, Julie Poulain, Arnaud Couloux, Kamel Jabbari, Federica Cattonaro, Cristian Del Fabbro, Sara Pinosio, Andrea Zuccolo, Jarrod Chapman, Jane Grimwood, Francisco R Tadeo, Leandro H Estornell, Juan V Mu, Victoria Ibanez, Amparo Herrero-Ortega, Pablo Aleza, Juli, Daniel Ram, Dominique Brunel, Fran, Chunxian Chen, William G Farmerie, Brian Desany, Chinnappa Kodira, Mohammed Mohiuddin, Tim Harkins, Karin Fredrikson, Paul Burns, Alexandre Lomsadze, Mark Borodovsky, Giuseppe Reforgiato, Juliana Freitas-Ast, Francis Quetier, Luis Navarro, Mikeal Roose, Patrick Wincker, Jeremy Schmutz, Michele Morgante, Marcos Antonio Machado, Manuel Talon, Olivier Jaillon, Patrick Ollitrault, Frederick Gmitter, Daniel Rokhsar

12) A unified test of linkage analysis and rare-variant association for analysis of pedigree sequence data (Nature Biotechnology) Sunday June 15th 2014
Hao Hu, Jared C Roach, Hilary Coon, Stephen L Guthery, Karl V Voelkerding, Rebecca L Margraf, Jacob D Durtschi, Sean V Tavtigian, Shankaracharya, Wilfred Wu, Paul Scheet, Shuoguo Wang, Jinchuan Xing, Gustavo Glusman, Robert Hubley, Hong Li, Vidu Garg, Barry Moore, Leroy Hood, David J Galas, Deepak Srivastava, Martin G Reese, Lynn B Jorde, Mark Yandell, Chad D Huff

13) Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease (Nature Biotechnology) Sunday June 15th 2014
Cem Kuscu, Sevki Arslan, Ritambhara Singh, Jeremy Thorpe, Mazhar Adli

14) A community effort to assess and improve drug sensitivity prediction algorithms (Nature Biotechnology) Sunday June 15th 2014
James C Costello, Laura M Heiser, Elisabeth Georgii, Mehmet G, Michael P Menden, Nicholas J Wang, Mukesh Bansal, Muhammad Ammad-ud-din, Petteri Hintsanen, Suleiman A Khan, John-Patrick Mpindi, Olli Kallioniemi, Antti Honkela, Tero Aittokallio, Krister Wennerberg, James J Collins, Dan Gallahan, Dinah Singer, Julio Saez-Rodriguez, Samuel Kaski, Joe W Gray, Gustavo Stolovitzky

15) Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification (Nature Biotechnology) Tuesday April 29th 2014
John P Guilinger, David B Thompson, David R Liu

16) Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing (Nature Biotechnology) Friday April 25th 2014
Shengdar Q Tsai, Nicolas Wyvekens, Cyd Khayter, Jennifer A Foden, Vishal Thapar, Deepak Reyon, Mathew J Goodwin, Martin J Aryee, J Keith Joung

17) Sailfish enables alignment-free isoform quantification from RNA-seq reads using lightweight algorithms (Nature Biotechnology) Sunday April 20th 2014
Rob Patro, Stephen M Mount, Carl Kingsford

18) Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells (Nature Biotechnology) Sunday April 20th 2014
Xuebing Wu, David A Scott, Andrea J Kriz, Anthony C Chiu, Patrick D Hsu, Daniel B Dadon, Albert W Cheng, Alexandro E Trevino, Silvana Konermann, Sidi Chen, Rudolf Jaenisch, Feng Zhang, Phillip A Sharp

19) Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor (Nature Biotechnology) Sunday April 20th 2014
Hyungsoon Im, Huilin Shao, Yong Il Park, Vanessa M Peterson, Cesar M Castro, Ralph Weissleder, Hakho Lee

20) Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer (Nature Biotechnology) Sunday April 20th 2014
Jens G Lohr, Viktor A Adalsteinsson, Kristian Cibulskis, Atish D Choudhury, Mara Rosenberg, Peter Cruz-Gordillo, Joshua M Francis, Cheng-Zhong Zhang, Alex K Shalek, Rahul Satija, John J Trombetta, Diana Lu, Naren Tallapragada, Narmin Tahirova, Sora Kim, Brendan Blumenstiel, Carrie Sougnez, Alarice Lowe, Bang Wong, Daniel Auclair, Eliezer M Van Allen, Mari Nakabayashi, Rosina T Lis, Gwo-Shu M Lee, Tiantian Li, Matthew S Chabot, Amy Ly, Mary-Ellen Taplin, Thomas E Clancy, Massimo Loda, Aviv Regev, Matthew Meyerson, William C Hahn, Philip W Kantoff, Todd R Golub, Gad Getz, Jesse S Boehm, J Christopher Love

21) GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins (Nature Biotechnology) Sunday April 20th 2014
Leander Meuris, Francis Santens, Greg Elson, Nele Festjens, Morgane Boone, Ana, Simon Devos, Fran, Evelyn Plets, Erica Houthuys, Pauline Malinge, Giovanni Magistrelli, Laura Cons, Laurence Chatel, Bart Devreese, Nico Callewaert

22) Quantitative analysis of RNA-protein interactions on a massively parallel array reveals biophysical and evolutionary landscapes (Nature Biotechnology) Monday April 14th 2014
Jason D Buenrostro, Carlos L Araya, Lauren M Chircus, Curtis J Layton, Howard Y Chang, Michael P Snyder, William J Greenleaf

23) Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal (Nature Biotechnology) Monday April 14th 2014
Thomas S Elliott, Fiona M Townsley, Ambra Bianco, Russell J Ernst, Amit Sachdeva, Simon J Els, Lloyd Davis, Kathrin Lang, Rudolf Pisa, Sebastian Greiss, Kathryn S Lilley, Jason W Chin

24) Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption (Nature Biotechnology) Monday April 7th 2014
Benjamin L Wang, Adel Ghaderi, Hang Zhou, Jeremy Agresti, David A Weitz, Gerald R Fink, Gregory Stephanopoulos

25) Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype (Nature Biotechnology) Sunday March 30th 2014
Hao Yin, Wen Xue, Sidi Chen, Roman L Bogorad, Eric Benedetti, Markus Grompe, Victor Koteliansky, Phillip A Sharp, Tyler Jacks, Daniel G Anderson

26) Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing (Nature Biotechnology) Sunday March 23rd 2014
Adam J Shuhendler, Kanyi Pu, Lina Cui, Jack P Uetrecht, Jianghong Rao

27) The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells (Nature Biotechnology) Sunday March 23rd 2014
Cole Trapnell, Davide Cacchiarelli, Jonna Grimsby, Prapti Pokharel, Shuqiang Li, Michael Morse, Niall J Lennon, Kenneth J Livak, Tarjei S Mikkelsen, John L Rinn

28) Development and function of human innate immune cells in a humanized mouse model (Nature Biotechnology) Monday March 17th 2014
Anthony Rongvaux, Tim Willinger, Jan Martinek, Till Strowig, Sofia V Gearty, Lino L Teichmann, Yasuyuki Saito, Florentina Marches, Stephanie Halene, A Karolina Palucka, Markus G Manz, Richard A Flavell

29) Massively parallel functional annotation of 3 (Nature Biotechnology) Monday March 17th 2014
Wenxue Zhao, Joshua L Pollack, Denitza P Blagev, Noah Zaitlen, Michael T McManus, David J Erle

30) Genome-guided transcript assembly by integrative analysis of RNA sequence data (Nature Biotechnology) Monday March 17th 2014
Nathan Boley, Marcus H Stoiber, Benjamin W Booth, Kenneth H Wan, Roger A Hoskins, Peter J Bickel, Susan E Celniker, James B Brown

31) CRISPR-Cas systems for editing, regulating and targeting genomes (Nature Biotechnology) Monday March 3rd 2014
Jeffry D Sander, J Keith Joung

32) Mesenchymal stem cells: immune evasive, not immune privileged (Nature Biotechnology) Sunday February 23rd 2014
James A Ankrum, Joon Faii Ong, Jeffrey M Karp

33) Whole-genome haplotyping using long reads and statistical methods (Nature Biotechnology) Sunday February 23rd 2014
Volodymyr Kuleshov, Dan Xie, Rui Chen, Dmitry Pushkarev, Zhihai Ma, Tim Blauwkamp, Michael Kertesz, Michael Snyder

34) The binary protein-protein interaction landscape of Escherichia coli (Nature Biotechnology) Sunday February 23rd 2014
Seesandra V Rajagopala, Patricia Sikorski, Ashwani Kumar, Roberto Mosca, James Vlasblom, Roland Arnold, Jonathan Franca-Koh, Suman B Pakala, Sadhna Phanse, Arnaud Ceol, Roman H, Gabriella Siszler, Stefan Wuchty, Andrew Emili, Mohan Babu, Patrick Aloy, Rembert Pieper, Peter Uetz

35) Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice (Nature Biotechnology) Sunday February 16th 2014
Shrivats Mohan Iyer, Kate L Montgomery, Chris Towne, Soo Yeun Lee, Charu Ramakrishnan, Karl Deisseroth, Scott L Delp

36) Integrating human sequence data sets provides a resource of benchmark SNP and indel genotype calls (Nature Biotechnology) Sunday February 16th 2014
Justin M Zook, Brad Chapman, Jason Wang, David Mittelman, Oliver Hofmann, Winston Hide, Marc Salit

37) Smac mimetics and innate immune stimuli synergize to promote tumor death (Nature Biotechnology) Sunday January 26th 2014
Shawn T Beug, Vera A Tang, Eric C LaCasse, Herman H Cheung, Caroline E Beauregard, Jan Brun, Jeffrey P Nuyens, Nathalie Earl, Martine St-Jean, Janelle Holbrook, Himika Dastidar, Douglas J Mahoney, Carolina Ilkow, Fabrice Le Boeuf, John C Bell, Robert G Korneluk

38) Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface (Nature Biotechnology) Sunday January 26th 2014
Steven M Lewis, Xiufeng Wu, Anna Pustilnik, Arlene Sereno, Flora Huang, Heather L Rick, Gurkan Guntas, Andrew Leaver-Fay, Eric M Smith, Carolyn Ho, Christophe Hansen-Estruch, Aaron K Chamberlain, Stephanie M Truhlar, Elaine M Conner, Shane Atwell, Brian Kuhlman, Stephen J Demarest

39) Improving CRISPR-Cas nuclease specificity using truncated guide RNAs (Nature Biotechnology) Sunday January 26th 2014
Yanfang Fu, Jeffry D Sander, Deepak Reyon, Vincent M Cascio, J Keith Joung

40) The promise and challenge of high-throughput sequencing of the antibody repertoire (Nature Biotechnology) Sunday January 19th 2014
George Georgiou, Gregory C Ippolito, John Beausang, Christian E Busse, Hedda Wardemann, Stephen R Quake

41) Beyond model antigens: high-dimensional methods for the analysis of antigen-specific T cells (Nature Biotechnology) Sunday January 19th 2014
Evan W Newell, Mark M Davis

42) Single-molecule site-specific detection of protein phosphorylation with a nanopore (Nature Biotechnology) Sunday January 19th 2014
Christian B Rosen, David Rodriguez-Larrea, Hagan Bayley

43) Computational resources for high-dimensional immune analysis from the Human Immunology Project Consortium (Nature Biotechnology) Sunday January 19th 2014
Vladimir Brusic, Raphael Gottardo, Steven H Kleinstein, Mark M Davis

44) New dimensions in immunology (Nature Biotechnology) Sunday January 19th 2014


45) Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library (Nature Biotechnology) Tuesday December 24th 2013
Hiroko Koike-Yusa, Yilong Li, E-Pien Tan, Martin Del Castillo Velasco-Herrera, Kosuke Yusa

46) Genome-wide localization of small molecules (Nature Biotechnology) Sunday December 15th 2013
Lars Anders, Matthew G Guenther, Jun Qi, Zi Peng Fan, Jason J Marineau, Peter B Rahl, Jakob Lov, Alla A Sigova, William B Smith, Tong Ihn Lee, James E Bradner, Richard A Young

47) Reduced local mutation density in regulatory DNA of cancer genomes is linked to DNA repair (Nature Biotechnology) Sunday December 15th 2013
Paz Polak, Michael S Lawrence, Eric Haugen, Nina Stoletzki, Petar Stojanov, Robert E Thurman, Levi A Garraway, Sergei Mirkin, Gad Getz, John A Stamatoyannopoulos, Shamil R Sunyaev

48) Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids (Nature Biotechnology) Monday December 9th 2013
Bryony C Bonning, Narinder Pal, Sijun Liu, Zhaohui Wang, S Sivakumar, Philip M Dixon, Glenn F King, W Allen Miller

49) High-throughput genome scaffolding from in vivo DNA interaction frequency (Nature Biotechnology) Monday November 25th 2013
Noam Kaplan, Job Dekker

50) Systematic comparison of phenome-wide association study of electronic medical record data and genome-wide association study data (Nature Biotechnology) Monday November 25th 2013
Joshua C Denny, Lisa Bastarache, Marylyn D Ritchie, Robert J Carroll, Raquel Zink, Jonathan D Mosley, Julie R Field, Jill M Pulley, Andrea H Ramirez, Erica Bowton, Melissa A Basford, David S Carrell, Peggy L Peissig, Abel N Kho, Jennifer A Pacheco, Luke V Rasmussen, David R Crosslin, Paul K Crane, Jyotishman Pathak, Suzette J Bielinski, Sarah A Pendergrass, Hua Xu, Lucia A Hindorff, Rongling Li, Teri A Manolio, Christopher G Chute, Rex L Chisholm, Eric B Larson, Gail P Jarvik, Murray H Brilliant, Catherine A McCarty, Iftikhar J Kullo, Jonathan L Haines, Dana C Crawford, Daniel R Masys, Dan M Roden

51) Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice (Nature Biotechnology) Monday November 18th 2013
Luc Baeyens, Marie Lemper, Gunter Leuckx, Sofie De Groef, Paola Bonfanti, Geert Stang, Ruth Shemer, Christoffer Nord, David W Scheel, Fong C Pan, Ulf Ahlgren, Guoqiang Gu, Doris A Stoffers, Yuval Dor, Jorge Ferrer, Gerard Gradwohl, Christopher V E Wright, Mark Van de Casteele, Michael S German, Luc Bouwens, Harry Heimberg

52) Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells (Nature Biotechnology) Sunday November 10th 2013
Jeff Gole, Athurva Gore, Andrew Richards, Yu-Jui Chiu, Ho-Lim Fung, Diane Bushman, Hsin-I Chiang, Jerold Chun, Yu-Hwa Lo, Kun Zhang

53) Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions (Nature Biotechnology) Sunday November 3rd 2013
Joshua N Burton, Andrew Adey, Rupali P Patwardhan, Ruolan Qiu, Jacob O Kitzman, Jay Shendure

54) Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing (Nature Biotechnology) Sunday November 3rd 2013
Siddarth Selvaraj, Jesse R Dixon, Vikas Bansal, Bing Ren

55) Directed evolution of genetic parts and circuits by compartmentalized partnered replication (Nature Biotechnology) Sunday November 3rd 2013
Jared W Ellefson, Adam J Meyer, Randall A Hughes, Joe R Cannon, Jennifer S Brodbelt, Andrew D Ellington

56) Reassessing target antigens for adoptive T-cell therapy (Nature Biotechnology) Sunday October 20th 2013
Christian S Hinrichs, Nicholas P Restifo

57) Engineering dynamic pathway regulation using stress-response promoters (Nature Biotechnology) Sunday October 20th 2013
Robert H Dahl, Fuzhong Zhang, Jorge Alonso-Gutierrez, Edward Baidoo, Tanveer S Batth, Alyssa M Redding-Johanson, Christopher J Petzold, Aindrila Mukhopadhyay, Taek Soon Lee, Paul D Adams, Jay D Keasling

58) Development and validation of a clinical cancer genomic profiling test based on massively parallel DNA sequencing (Nature Biotechnology) Sunday October 20th 2013
Garrett M Frampton, Alex Fichtenholtz, Geoff A Otto, Kai Wang, Sean R Downing, Jie He, Michael Schnall-Levin, Jared White, Eric M Sanford, Peter An, James Sun, Frank Juhn, Kristina Brennan, Kiel Iwanik, Ashley Maillet, Jamie Buell, Emily White, Mandy Zhao, Sohail Balasubramanian, Selmira Terzic, Tina Richards, Vera Banning, Lazaro Garcia, Kristen Mahoney, Zac Zwirko, Amy Donahue, Himisha Beltran, Juan Miguel Mosquera, Mark A Rubin, Snjezana Dogan, Cyrus V Hedvat, Michael F Berger, Lajos Pusztai, Matthias Lechner, Chris Boshoff, Mirna Jarosz, Christine Vietz, Alex Parker, Vincent A Miller, Jeffrey S Ross, John Curran, Maureen T Cronin, Philip J Stephens, Doron Lipson, Roman Yelensky

59) A single-molecule long-read survey of the human transcriptome (Nature Biotechnology) Wednesday October 9th 2013
Donald Sharon, Hagen Tilgner, Fabian Grubert, Michael Snyder

60) Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy (Nature Biotechnology) Wednesday October 9th 2013
Yicong Wu, Peter Wawrzusin, Justin Senseney, Robert S Fischer, Ryan Christensen, Anthony Santella, Andrew G York, Peter W Winter, Clare M Waterman, Zhirong Bao, Daniel A Col, Matthew McAuliffe, Hari Shroff

61) Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins (Nature Biotechnology) Wednesday October 9th 2013
Morgan L Maeder, James F Angstman, Marcy E Richardson, Samantha J Linder, Vincent M Cascio, Shengdar Q Tsai, Quan H Ho, Jeffry D Sander, Deepak Reyon, Bradley E Bernstein, Joseph F Costello, Miles F Wilkinson, J Keith Joung

62) Strategic addition of an N-linked glycan to a monoclonal antibody improves its HIV-1 (Nature Biotechnology) Sunday October 6th 2013
Ruijiang Song, Deena A Oren, David Franco, Michael S Seaman, David D Ho

63) Identifying producers of antibacterial compounds by screening for antibiotic resistance (Nature Biotechnology) Sunday October 6th 2013
Maulik N Thaker, Wenliang Wang, Peter Spanogiannopoulos, Nicholas Waglechner, Andrew M King, Ricardo Medina, Gerard D Wright

64) Accelerating the design of biomimetic materials by integrating RNA-seq with proteomics and materials science (Nature Biotechnology) Sunday September 8th 2013
Paul A Guerette, Shawn Hoon, Yiqi Seow, Manfred Raida, Admir Masic, Fong T Wong, Vincent H B Ho, Kiat Whye Kong, Melik C Demirel, Abdon Pena-Francesch, Shahrouz Amini, Gavin Z Tay, Dawei Ding, Ali Miserez

65) Modified mRNA directs the fate of heart progenitor cells and induces vascular regeneration after myocardial infarction (Nature Biotechnology) Sunday September 8th 2013
Lior Zangi, Kathy O Lui, Alexander von Gise, Qing Ma, Wataru Ebina, Leon M Ptaszek, Daniela Sp, Huansheng Xu, Mohammadsharif Tabebordbar, Rostic Gorbatov, Brena Sena, Matthias Nahrendorf, David M Briscoe, Ronald A Li, Amy J Wagers, Derrick J Rossi, William T Pu, Kenneth R Chien

66) Locus-specific editing of histone modifications at endogenous enhancers (Nature Biotechnology) Sunday September 8th 2013
Eric M Mendenhall, Kaylyn E Williamson, Deepak Reyon, James Y Zou, Oren Ram, J Keith Joung, Bradley E Bernstein

67) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences (Nature Biotechnology) Sunday August 25th 2013
Morgan G I Langille, Jesse Zaneveld, J Gregory Caporaso, Daniel McDonald, Dan Knights, Joshua A Reyes, Jose C Clemente, Deron E Burkepile, Rebecca L Vega Thurber, Rob Knight, Robert G Beiko, Curtis Huttenhower

68) Nature-inspired design of motif-specific antibody scaffolds (Nature Biotechnology) Monday August 19th 2013
James T Koerber, Nathan D Thomsen, Brett T Hannigan, William F Degrado, James A Wells

69) Conditional targeted genome editing using somatically expressed TALENs in C. elegans (Nature Biotechnology) Monday August 19th 2013
Ze Cheng, Peishan Yi, Xiangming Wang, Yongping Chai, Guoxin Feng, Yihong Yang, Xing Liang, Zhiwen Zhu, Wei Li, Guangshuo Ou

70) MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies (Nature Biotechnology) Sunday August 11th 2013
Ryan A Langlois, Randy A Albrecht, Brian Kimble, Troy Sutton, Jillian S Shapiro, Courtney Finch, Matthew Angel, Mark A Chua, Ana Silvia Gonzalez-Reiche, Kemin Xu, Daniel Perez, Adolfo Garc, Benjamin R tenOever

71) High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity (Nature Biotechnology) Sunday August 11th 2013
Vikram Pattanayak, Steven Lin, John P Guilinger, Enbo Ma, Jennifer A Doudna, David R Liu

72) Generation of tumor-targeted human T lymphocytes from induced pluripotent stem cells for cancer therapy (Nature Biotechnology) Sunday August 11th 2013
Maria Themeli, Christopher C Kloss, Giovanni Ciriello, Victor D Fedorov, Fabiana Perna, Mithat Gonen, Michel Sadelain

73) CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering (Nature Biotechnology) Thursday August 1st 2013
Prashant Mali, John Aach, P Benjamin Stranges, Kevin M Esvelt, Mark Moosburner, Sriram Kosuri, Luhan Yang, George M Church

74) Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica (Nature Biotechnology) Sunday July 21st 2013
Zhixiong Xue, Pamela L Sharpe, Seung-Pyo Hong, Narendra S Yadav, Dongming Xie, David R Short, Howard G Damude, Ross A Rupert, John E Seip, Jamie Wang, Dana W Pollak, Michael W Bostick, Melissa D Bosak, Daniel J Macool, Dieter H Hollerbach, Hongxiang Zhang, Dennis M Arcilla, Sidney A Bledsoe, Kevin Croker, Elizabeth F McCord, Bjorn D Tyreus, Ethel N Jackson, Quinn Zhu

75) Photoreceptor precursors derived from three-dimensional embryonic stem cell cultures integrate and mature within adult degenerate retina (Nature Biotechnology) Sunday July 21st 2013
Anai Gonzalez-Cordero, Emma L West, Rachael A Pearson, Yanai Duran, Livia S Carvalho, Colin J Chu, Arifa Naeem, Samuel J I Blackford, Anastasios Georgiadis, Jorn Lakowski, Mike Hubank, Alexander J Smith, James W B Bainbridge, Jane C Sowden, Robin R Ali

76) DNA targeting specificity of RNA-guided Cas9 nucleases (Nature Biotechnology) Sunday July 21st 2013
Patrick D Hsu, David A Scott, Joshua A Weinstein, F Ann Ran, Silvana Konermann, Vineeta Agarwala, Yinqing Li, Eli J Fine, Xuebing Wu, Ophir Shalem, Thomas J Cradick, Luciano A Marraffini, Gang Bao, Feng Zhang

77) Single-cell gene expression analysis reveals genetic associations masked in whole-tissue experiments (Nature Biotechnology) Sunday July 21st 2013
Quin F Wills, Kenneth J Livak, Alex J Tipping, Tariq Enver, Andrew J Goldson, Darren W Sexton, Chris Holmes

78) Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching (Nature Biotechnology) Sunday July 21st 2013
Yu-Chan Zhang, Yang Yu, Cong-Ying Wang, Ze-Yuan Li, Qing Liu, Jie Xu, Jian-You Liao, Xiao-Jing Wang, Liang-Hu Qu, Fan Chen, Peiyong Xin, Cunyu Yan, Jinfang Chu, Hong-Qing Li, Yue-Qin Chen

79) Network link prediction by global silencing of indirect correlations (Nature Biotechnology) Sunday July 14th 2013
Baruch Barzel, Albert-L

80) Network deconvolution as a general method to distinguish direct dependencies in networks (Nature Biotechnology) Sunday July 14th 2013
Soheil Feizi, Daniel Marbach, Muriel M, Manolis Kellis

81) Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies (Nature Biotechnology) Sunday July 7th 2013
Christoph Spiess, Mark Merchant, Arthur Huang, Zhong Zheng, Nai-Ying Yang, Jing Peng, Diego Ellerman, Whitney Shatz, Dorothea Reilly, Daniel G Yansura, Justin M Scheer

82) Chaperones as thermodynamic sensors of drug-target interactions reveal kinase inhibitor specificities in living cells (Nature Biotechnology) Sunday June 30th 2013
Mikko Taipale, Irina Krykbaeva, Luke Whitesell, Sandro Santagata, Jianming Zhang, Qingsong Liu, Nathanael S Gray, Susan Lindquist

83) Image-based analysis of lipid nanoparticle (Nature Biotechnology) Monday June 24th 2013
Jerome Gilleron, William Querbes, Anja Zeigerer, Anna Borodovsky, Giovanni Marsico, Undine Schubert, Kevin Manygoats, Sarah Seifert, Cordula Andree, Martin St, Hila Epstein-Barash, Ligang Zhang, Victor Koteliansky, Kevin Fitzgerald, Eugenio Fava, Marc Bickle, Yannis Kalaidzidis, Akin Akinc, Martin Maier, Marino Zerial

84) High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells (Nature Biotechnology) Monday June 24th 2013
Yanfang Fu, Jennifer A Foden, Cyd Khayter, Morgan L Maeder, Deepak Reyon, J Keith Joung, Jeffry D Sander

85) Efficiency of siRNA delivery by lipid nanoparticles is limited by endocytic recycling (Nature Biotechnology) Monday June 24th 2013
Gaurav Sahay, William Querbes, Christopher Alabi, Ahmed Eltoukhy, Sovan Sarkar, Christopher Zurenko, Emmanouil Karagiannis, Kevin Love, Delai Chen, Roberto Zoncu, Yosef Buganim, Avi Schroeder, Robert Langer, Daniel G Anderson

86) Uniform, optimal signal processing of mapped deep-sequencing data (Nature Biotechnology) Monday June 17th 2013
Vibhor Kumar, Masafumi Muratani, Nirmala Arul Rayan, Petra Kraus, Thomas Lufkin, Huck Hui Ng, Shyam Prabhakar

87) Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization (Nature Biotechnology) Sunday June 9th 2013
Evan W Newell, Natalia Sigal, Nitya Nair, Brian A Kidd, Harry B Greenberg, Mark M Davis

88) Broad protection against influenza infection by vectored immunoprophylaxis in mice (Nature Biotechnology) Sunday June 2nd 2013
Alejandro B Balazs, Jesse D Bloom, Christin M Hong, Dinesh S Rao, David Baltimore

89) Genome sequences of rare, uncultured bacteria obtained by differential coverage binning of multiple metagenomes (Nature Biotechnology) Sunday May 26th 2013
Mads Albertsen, Philip Hugenholtz, Adam Skarshewski, K, Gene W Tyson, Per H Nielsen

90) A large synthetic peptide and phosphopeptide reference library for mass spectrometry (Nature Biotechnology) Sunday May 19th 2013
Harald Marx, Simone Lemeer, Jan Erik Schliep, Lucrece Matheron, Shabaz Mohammed, J, Matthias Mann, Albert J R Heck, Bernhard Kuster

91) TALEN-mediated editing of the mouse Y chromosome (Nature Biotechnology) Sunday May 12th 2013
Haoyi Wang, Yueh-Chiang Hu, Styliani Markoulaki, G Grant Welstead, Albert W Cheng, Chikdu S Shivalila, Tatyana Pyntikova, Daniel B Dadon, Daniel F Voytas, Adam J Bogdanove, David C Page, Rudolf Jaenisch

92) Zwitterionic hydrogels implanted in mice resist the foreign-body reaction (Nature Biotechnology) Sunday May 12th 2013
Lei Zhang, Zhiqiang Cao, Tao Bai, Louisa Carr, Jean-Rene Ella-Menye, Colleen Irvin, Buddy D Ratner, Shaoyi Jiang

93) Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay (Nature Biotechnology) Sunday April 21st 2013
Ir, Andreas Schneeweiss, Sabine Riethdorf, Albrecht Stenzinger, Anja Schillert, Vanessa Vogel, Corinna Klein, Massimo Saini, Tobias B, Markus Wallwiener, Tim Holland-Letz, Thomas H, Martin Sprick, Martina Scharpff, Frederik Marm, Hans Peter Sinn, Klaus Pantel, Wilko Weichert, Andreas Trumpp

94) Medial ganglionic eminence (Nature Biotechnology) Sunday April 21st 2013
Yan Liu, Jason P Weick, Huisheng Liu, Robert Krencik, Xiaoqing Zhang, Lixiang Ma, Guo-min Zhou, Melvin Ayala, Su-Chun Zhang

95) Identification of direct targets and modified bases of RNA cytosine methyltransferases (Nature Biotechnology) Sunday April 21st 2013
Vahid Khoddami, Bradley R Cairns

96) Transcription factor (Nature Biotechnology) Sunday April 14th 2013
Fadi J Najm, Angela M Lager, Anita Zaremba, Krysta Wyatt, Andrew V Caprariello, Daniel C Factor, Robert T Karl, Tadao Maeda, Robert H Miller, Paul J Tesar

97) Generation of oligodendroglial cells by direct lineage conversion (Nature Biotechnology) Sunday April 14th 2013
Nan Yang, J Bradley Zuchero, Henrik Ahlenius, Samuele Marro, Yi Han Ng, Thomas Vierbuchen, John S Hawkins, Richard Geissler, Ben A Barres, Marius Wernig

98) Generation of high-affinity DNA aptamers using an expanded genetic alphabet (Nature Biotechnology) Sunday April 7th 2013
Michiko Kimoto, Rie Yamashige, Ken-ichiro Matsunaga, Shigeyuki Yokoyama, Ichiro Hirao

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