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1) Global analysis of protein structural changes in complex proteomes (Nature Biotechnology) Sunday September 14th 2014
Yuehan Feng, Giorgia De Franceschi, Abdullah Kahraman, Martin Soste, Andre Melnik, Paul J Boersema, Patrizia Polverino de Laureto, Yaroslav Nikolaev, Ana Paula Oliveira, Paola Picotti

2) De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits (Nature Biotechnology) Sunday September 14th 2014
Ying-hui Li, Guangyu Zhou, Jianxin Ma, Wenkai Jiang, Long-guo Jin, Zhouhao Zhang, Yong Guo, Jinbo Zhang, Yi Sui, Liangtao Zheng, Shan-shan Zhang, Qiyang Zuo, Xue-hui Shi, Yan-fei Li, Wan-ke Zhang, Yiyao Hu, Guanyi Kong, Hui-long Hong, Bing Tan, Jian Song, Zhang-xiong Liu, Yaoshen Wang, Hang Ruan, Carol K L Yeung, Jian Liu, Hailong Wang, Li-juan Zhang, Rong-xia Guan, Ke-jing Wang, Wen-bin Li, Shou-yi Chen, Ru-zhen Chang, Zhi Jiang, Scott A Jackson, Ruiqiang Li, Li-juan Qiu

3) Self-hydrolyzing maleimides improve the stability and pharmacological properties of antibody-drug conjugates (Nature Biotechnology) Sunday September 7th 2014
Robert P Lyon, Jocelyn R Setter, Tim D Bovee, Svetlana O Doronina, Joshua H Hunter, Martha E Anderson, Cindy L Balasubramanian, Steven M Duniho, Chris I Leiske, Fu Li, Peter D Senter

4) Rational design of highly active sgRNAs for CRISPR-Cas9 (Nature Biotechnology) Wednesday September 3rd 2014
John G Doench, Ella Hartenian, Daniel B Graham, Zuzana Tothova, Mudra Hegde, Ian Smith, Meagan Sullender, Benjamin L Ebert, Ramnik J Xavier, David E Root

5) Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study (Nature Biotechnology) Sunday August 24th 2014
Sheng Li, Scott W Tighe, Charles M Nicolet, Deborah Grove, Shawn Levy, William Farmerie, Agnes Viale, Chris Wright, Peter A Schweitzer, Yuan Gao, Dewey Kim, Joe Boland, Belynda Hicks, Ryan Kim, Sagar Chhangawala, Nadereh Jafari, Nalini Raghavachari, Jorge Gandara, Nat, Cynthia Hendrickson, David Roberson, Jeffrey Rosenfeld, Todd Smith, Jason G Underwood, May Wang, Paul Zumbo, Don A Baldwin, George S Grills, Christopher E Mason

6) A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequencing Quality Control Consortium (Nature Biotechnology) Sunday August 24th 2014
Zhenqiang Su, Pawe, Sheng Li, Jean Thierry-Mieg, Danielle Thierry-Mieg, Wei Shi, Charles Wang, Gary P Schroth, Robert A Setterquist, John F Thompson, Wendell D Jones, Wenzhong Xiao, Weihong Xu, Roderick V Jensen, Reagan Kelly, Joshua Xu, Ana Conesa, Cesare Furlanello, Hanlin Gao, Huixiao Hong, Nadereh Jafari, Stan Letovsky, Yang Liao, Fei Lu, Edward J Oakeley, Zhiyu Peng, Craig A Praul, Javier Santoyo-Lopez, Andreas Scherer, Tieliu Shi, Gordon K Smyth, Frank Staedtler, Peter Sykacek, Xin-Xing Tan, E Aubrey Thompson, Jo Vandesompele, May D Wang, Jian Wang, Russell D Wolfinger, Jiri Zavadil, Scott S Auerbach, Wenjun Bao, Hans Binder, Thomas Blomquist, Murray H Brilliant, Pierre R Bushel, Weimin Cai, Jennifer G Catalano, Ching-Wei Chang, Tao Chen, Geng Chen, Rong Chen, Marco Chierici, Tzu-Ming Chu, Djork-Arn, Youping Deng, Adnan Derti, Viswanath Devanarayan, Zirui Dong, Joaquin Dopazo, Tingting Du, Hong Fang, Yongxiang Fang, Mario Fasold, Anita Fernandez, Matthias Fischer, Pedro Furi, James C Fuscoe, Florian Caimet, Stan Gaj, Jorge Gandara, Huan Gao, Weigong Ge, Yoichi Gondo, Binsheng Gong, Meihua Gong, Zhuolin Gong, Bridgett Green, Chao Guo, Lei Guo, Li-Wu Guo, James Hadfield, Jan Hellemans, Sepp Hochreiter, Meiwen Jia, Min Jian, Charles D Johnson, Suzanne Kay, Jos Kleinjans, Samir Lababidi, Shawn Levy, Quan-Zhen Li, Li Li, Li Li, Peng Li, Yan Li, Haiqing Li, Jianying Li, Shiyong Li, Simon M Lin, Francisco J L, Xin Lu, Heng Luo, Xiwen Ma, Joseph Meehan, Dalila B Megherbi, Nan Mei, Bing Mu, Baitang Ning, Akhilesh Pandey, Javier P, Roger G Perkins, Ryan Peters, John H Phan, Mehdi Pirooznia, Feng Qian, Tao Qing, Lucille Rainbow, Philippe Rocca-Serra, Laure Sambourg, Susanna-Assunta Sansone, Scott Schwartz, Ruchir Shah, Jie Shen, Todd M Smith, Oliver Stegle, Nancy Stralis-Pavese, Elia Stupka, Yutaka Suzuki, Lee T Szkotnicki, Matthew Tinning, Bimeng Tu, Joost van Delft, Alicia Vela-Boza, Elisa Venturini, Stephen J Walker, Liqing Wan, Wei Wang, Jinhui Wang, Jun Wang, Eric D Wieben, James C Willey, Po-Yen Wu, Jiekun Xuan, Yong Yang, Zhan Ye, Ye Yin, Ying Yu, Yate-Ching Yuan, John Zhang, Ke K Zhang, Wenqian Zhang, Wenwei Zhang, Yanyan Zhang, Chen Zhao, Yuanting Zheng, Yiming Zhou, Paul Zumbo, Weida Tong, David P Kreil, Christopher E Mason, Leming Shi

7) The concordance between RNA-seq and microarray data depends on chemical treatment and transcript abundance (Nature Biotechnology) Sunday August 24th 2014
Charles Wang, Binsheng Gong, Pierre R Bushel, Jean Thierry-Mieg, Danielle Thierry-Mieg, Joshua Xu, Hong Fang, Huixiao Hong, Jie Shen, Zhenqiang Su, Joe Meehan, Xiaojin Li, Lu Yang, Haiqing Li, Pawe, David P Kreil, Dalila Megherbi, Stan Gaj, Florian Caiment, Joost van Delft, Jos Kleinjans, Andreas Scherer, Viswanath Devanarayan, Jian Wang, Yong Yang, Hui-Rong Qian, Lee J Lancashire, Marina Bessarabova, Yuri Nikolsky, Cesare Furlanello, Marco Chierici, Davide Albanese, Giuseppe Jurman, Samantha Riccadonna, Michele Filosi, Roberto Visintainer, Ke K Zhang, Jianying Li, Jui-Hua Hsieh, Daniel L Svoboda, James C Fuscoe, Youping Deng, Leming Shi, Richard S Paules, Scott S Auerbach, Weida Tong

8) Detecting and correcting systematic variation in large-scale RNA sequencing data (Nature Biotechnology) Sunday August 24th 2014
Sheng Li, Pawe, Paul Zumbo, Peter Sykacek, Wei Shi, Leming Shi, John Phan, Po-Yen Wu, May Wang, Charles Wang, Danielle Thierry-Mieg, Jean Thierry-Mieg, David P Kreil, Christopher E Mason

9) Normalization of RNA-seq data using factor analysis of control genes or samples (Nature Biotechnology) Sunday August 24th 2014
Davide Risso, John Ngai, Terence P Speed, Sandrine Dudoit

10) Targeted sequencing by proximity ligation for comprehensive variant detection and local haplotyping (Nature Biotechnology) Sunday August 17th 2014
Paula J P de Vree, Elzo de Wit, Mehmet Yilmaz, Monique van de Heijning, Petra Klous, Marjon J A M Verstegen, Yi Wan, Hans Teunissen, Peter H L Krijger, Geert Geeven, Paul P Eijk, Daoud Sie, Bauke Ylstra, Lorette O M Hulsman, Marieke F van Dooren, Laura J C M van Zutven, Ans van den Ouweland, Sjef Verbeek, Ko Willems van Dijk, Marion Cornelissen, Atze T Das, Ben Berkhout, Birgit Sikkema-Raddatz, Eva van den Berg, Pieter van der Vlies, Desiree Weening, Johan T den Dunnen, Magdalena Matusiak, Mohamed Lamkanfi, Marjolijn J L Ligtenberg, Petra ter Brugge, Jos Jonkers, John A Foekens, John W Martens, Rob van der Luijt, Hans Kristian Ploos van Amstel, Max van Min, Erik Splinter, Wouter de Laat

11) Microfluidic organs-on-chips (Nature Biotechnology) Tuesday August 5th 2014
Sangeeta N Bhatia, Donald E Ingber

12) Modulating the stem cell niche for tissue regeneration (Nature Biotechnology) Tuesday August 5th 2014
Steven W Lane, David A Williams, Fiona M Watt

13) 3D bioprinting of tissues and organs (Nature Biotechnology) Tuesday August 5th 2014
Sean V Murphy, Anthony Atala

14) Clinical imaging in regenerative medicine (Nature Biotechnology) Tuesday August 5th 2014
Anna V Naumova, Michel Modo, Anna Moore, Charles E Murry, Joseph A Frank

15) Overcoming immunological barriers in regenerative medicine (Nature Biotechnology) Tuesday August 5th 2014
Johannes L Zakrzewski, Marcel R M van den Brink, Jeffrey A Hubbell

16) Harmonizing standards for producing clinical-grade therapies from pluripotent stem cells (Nature Biotechnology) Tuesday August 5th 2014
Peter W Andrews, Joy Cavanagro, Robert Deans, Ellen Feigel, Ed Horowitz, Armand Keating, Mahendra Rao, Marc Turner, Ian Wilmut, Shinya Yamanaka

17) An FDA perspective on preclinical development of cell-based regenerative medicine products (Nature Biotechnology) Tuesday August 5th 2014
Alexander M Bailey, Michael Mendicino, Patrick Au

18) Between disease and a dish (Nature Biotechnology) Tuesday August 5th 2014
Ken Garber

19) The global intellectual property landscape of induced pluripotent stem cell technologies (Nature Biotechnology) Tuesday August 5th 2014
MacKenna Roberts, Ivan B Wall, Ian Bingham, Dominic Icely, Brock Reeve, Kim Bure, Anna French, David A Brindley

20) Therapies of the state (Nature Biotechnology) Tuesday August 5th 2014
Beth Schachter

21) Cell presses (Nature Biotechnology) Tuesday August 5th 2014
Gunjan Sinha

22) Rethinking clinical delivery of adult stem cell therapies (Nature Biotechnology) Tuesday August 5th 2014
Nuala Trainor, Alexis Pietak, Tim Smith

23) Recent patent applications in stem cell reprogramming (Nature Biotechnology) Tuesday August 5th 2014


24) Back to the future (Nature Biotechnology) Tuesday August 5th 2014


25) Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex (Nature Biotechnology) Sunday August 3rd 2014
Alex A Pollen, Tomasz J Nowakowski, Joe Shuga, Xiaohui Wang, Anne A Leyrat, Jan H Lui, Nianzhen Li, Lukasz Szpankowski, Brian Fowler, Peilin Chen, Naveen Ramalingam, Gang Sun, Myo Thu, Michael Norris, Ronald Lebofsky, Dominique Toppani, Darnell W Kemp, Michael Wong, Barry Clerkson, Brittnee N Jones, Shiquan Wu, Lawrence Knutsson, Beatriz Alvarado, Jing Wang, Lesley S Weaver, Andrew P May, Robert C Jones, Marc A Unger, Arnold R Kriegstein, Jay A A West

26) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew (Nature Biotechnology) Sunday July 20th 2014
Yanpeng Wang, Xi Cheng, Qiwei Shan, Yi Zhang, Jinxing Liu, Caixia Gao, Jin-Long Qiu

27) Identification and assembly of genomes and genetic elements in complex metagenomic samples without using reference genomes (Nature Biotechnology) Sunday July 6th 2014
H Bj, Mathieu Almeida, Agnieszka Sierakowska Juncker, Simon Rasmussen, Junhua Li, Shinichi Sunagawa, Damian R Plichta, Laurent Gautier, Anders G Pedersen, Emmanuelle Le Chatelier, Eric Pelletier, Ida Bonde, Trine Nielsen, Chaysavanh Manichanh, Manimozhiyan Arumugam, Jean-Michel Batto, Marcelo B Quintanilha dos Santos, Nikolaj Blom, Natalia Borruel, Kristoffer S Burgdorf, Fouad Boumezbeur, Francesc Casellas, Jo, Piotr Dworzynski, Francisco Guarner, Torben Hansen, Falk Hildebrand, Rolf S Kaas, Sean Kennedy, Karsten Kristiansen, Jens Roat Kultima, Pierre L, Florence Levenez, Ole Lund, Bouziane Moumen, Denis Le Paslier, Nicolas Pons, Oluf Pedersen, Edi Prifti, Junjie Qin, Jeroen Raes, S, Julien Tap, Sebastian Tims, David W Ussery, Takuji Yamada, Pierre Renault, Thomas Sicheritz-Ponten, Peer Bork, Jun Wang, S, S Dusko Ehrlich

28) Revealing long noncoding RNA architecture and functions using domain-specific chromatin isolation by RNA purification (Nature Biotechnology) Sunday July 6th 2014
Jeffrey J Quinn, Ibrahim A Ilik, Kun Qu, Plamen Georgiev, Ci Chu, Asifa Akhtar, Howard Y Chang

29) An integrated catalog of reference genes in the human gut microbiome (Nature Biotechnology) Sunday July 6th 2014
Junhua Li, Huijue Jia, Xianghang Cai, Huanzi Zhong, Qiang Feng, Shinichi Sunagawa, Manimozhiyan Arumugam, Jens Roat Kultima, Edi Prifti, Trine Nielsen, Agnieszka Sierakowska Juncker, Chaysavanh Manichanh, Bing Chen, Wenwei Zhang, Florence Levenez, Juan Wang, Xun Xu, Liang Xiao, Suisha Liang, Dongya Zhang, Zhaoxi Zhang, Weineng Chen, Hailong Zhao, Jumana Yousuf Al-Aama, Sherif Edris, Huanming Yang, Jian Wang, Torben Hansen, Henrik Bj, S, Karsten Kristiansen, Francisco Guarner, Oluf Pedersen, Joel Dor, S Dusko Ehrlich, Peer Bork, Jun Wang

30) Decoding long nanopore sequencing reads of natural DNA (Nature Biotechnology) Wednesday June 25th 2014
Andrew H Laszlo, Ian M Derrington, Brian C Ross, Henry Brinkerhoff, Andrew Adey, Ian C Nova, Jonathan M Craig, Kyle W Langford, Jenny Mae Samson, Riza Daza, Kenji Doering, Jay Shendure, Jens H Gundlach

31) Linking T-cell receptor sequence to functional phenotype at the single-cell level (Nature Biotechnology) Sunday June 22nd 2014
Arnold Han, Jacob Glanville, Leo Hansmann, Mark M Davis

32) Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing (Nature Biotechnology) Sunday June 22nd 2014
Dirk Heckl, Monika S Kowalczyk, David Yudovich, Roger Belizaire, Rishi V Puram, Marie E McConkey, Anne Thielke, Jon C Aster, Aviv Regev, Benjamin L Ebert

33) Assessing the clinical utility of cancer genomic and proteomic data across tumor types (Nature Biotechnology) Sunday June 22nd 2014
Yuan Yuan, Eliezer M Van Allen, Larsson Omberg, Nikhil Wagle, Ali Amin-Mansour, Artem Sokolov, Lauren A Byers, Yanxun Xu, Kenneth R Hess, Lixia Diao, Leng Han, Xuelin Huang, Michael S Lawrence, John N Weinstein, Josh M Stuart, Gordon B Mills, Levi A Garraway, Adam A Margolin, Gad Getz, Han Liang

34) Backbone modification of a polypeptide drug alters duration of action in vivo (Nature Biotechnology) Sunday June 15th 2014
Ross W Cheloha, Akira Maeda, Thomas Dean, Thomas J Gardella, Samuel H Gellman

35) Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system (Nature Biotechnology) Sunday June 15th 2014
Mehdi Ghorbal, Molly Gorman, Cameron Ross Macpherson, Rafael Miyazawa Martins, Artur Scherf, Jose-Juan Lopez-Rubio

36) Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication (Nature Biotechnology) Sunday June 15th 2014
G Albert Wu, Simon Prochnik, Jerry Jenkins, Jerome Salse, Uffe Hellsten, Florent Murat, Xavier Perrier, Manuel Ruiz, Simone Scalabrin, Javier Terol, Marco Aur, Karine Labadie, Julie Poulain, Arnaud Couloux, Kamel Jabbari, Federica Cattonaro, Cristian Del Fabbro, Sara Pinosio, Andrea Zuccolo, Jarrod Chapman, Jane Grimwood, Francisco R Tadeo, Leandro H Estornell, Juan V Mu, Victoria Ibanez, Amparo Herrero-Ortega, Pablo Aleza, Juli, Daniel Ram, Dominique Brunel, Fran, Chunxian Chen, William G Farmerie, Brian Desany, Chinnappa Kodira, Mohammed Mohiuddin, Tim Harkins, Karin Fredrikson, Paul Burns, Alexandre Lomsadze, Mark Borodovsky, Giuseppe Reforgiato, Juliana Freitas-Ast, Francis Quetier, Luis Navarro, Mikeal Roose, Patrick Wincker, Jeremy Schmutz, Michele Morgante, Marcos Antonio Machado, Manuel Talon, Olivier Jaillon, Patrick Ollitrault, Frederick Gmitter, Daniel Rokhsar

37) A unified test of linkage analysis and rare-variant association for analysis of pedigree sequence data (Nature Biotechnology) Sunday June 15th 2014
Hao Hu, Jared C Roach, Hilary Coon, Stephen L Guthery, Karl V Voelkerding, Rebecca L Margraf, Jacob D Durtschi, Sean V Tavtigian, Shankaracharya, Wilfred Wu, Paul Scheet, Shuoguo Wang, Jinchuan Xing, Gustavo Glusman, Robert Hubley, Hong Li, Vidu Garg, Barry Moore, Leroy Hood, David J Galas, Deepak Srivastava, Martin G Reese, Lynn B Jorde, Mark Yandell, Chad D Huff

38) Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease (Nature Biotechnology) Sunday June 15th 2014
Cem Kuscu, Sevki Arslan, Ritambhara Singh, Jeremy Thorpe, Mazhar Adli

39) A community effort to assess and improve drug sensitivity prediction algorithms (Nature Biotechnology) Sunday June 15th 2014
James C Costello, Laura M Heiser, Elisabeth Georgii, Mehmet G, Michael P Menden, Nicholas J Wang, Mukesh Bansal, Muhammad Ammad-ud-din, Petteri Hintsanen, Suleiman A Khan, John-Patrick Mpindi, Olli Kallioniemi, Antti Honkela, Tero Aittokallio, Krister Wennerberg, James J Collins, Dan Gallahan, Dinah Singer, Julio Saez-Rodriguez, Samuel Kaski, Joe W Gray, Gustavo Stolovitzky

40) Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification (Nature Biotechnology) Tuesday April 29th 2014
John P Guilinger, David B Thompson, David R Liu

41) Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing (Nature Biotechnology) Friday April 25th 2014
Shengdar Q Tsai, Nicolas Wyvekens, Cyd Khayter, Jennifer A Foden, Vishal Thapar, Deepak Reyon, Mathew J Goodwin, Martin J Aryee, J Keith Joung

42) Sailfish enables alignment-free isoform quantification from RNA-seq reads using lightweight algorithms (Nature Biotechnology) Sunday April 20th 2014
Rob Patro, Stephen M Mount, Carl Kingsford

43) Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells (Nature Biotechnology) Sunday April 20th 2014
Xuebing Wu, David A Scott, Andrea J Kriz, Anthony C Chiu, Patrick D Hsu, Daniel B Dadon, Albert W Cheng, Alexandro E Trevino, Silvana Konermann, Sidi Chen, Rudolf Jaenisch, Feng Zhang, Phillip A Sharp

44) Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor (Nature Biotechnology) Sunday April 20th 2014
Hyungsoon Im, Huilin Shao, Yong Il Park, Vanessa M Peterson, Cesar M Castro, Ralph Weissleder, Hakho Lee

45) Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer (Nature Biotechnology) Sunday April 20th 2014
Jens G Lohr, Viktor A Adalsteinsson, Kristian Cibulskis, Atish D Choudhury, Mara Rosenberg, Peter Cruz-Gordillo, Joshua M Francis, Cheng-Zhong Zhang, Alex K Shalek, Rahul Satija, John J Trombetta, Diana Lu, Naren Tallapragada, Narmin Tahirova, Sora Kim, Brendan Blumenstiel, Carrie Sougnez, Alarice Lowe, Bang Wong, Daniel Auclair, Eliezer M Van Allen, Mari Nakabayashi, Rosina T Lis, Gwo-Shu M Lee, Tiantian Li, Matthew S Chabot, Amy Ly, Mary-Ellen Taplin, Thomas E Clancy, Massimo Loda, Aviv Regev, Matthew Meyerson, William C Hahn, Philip W Kantoff, Todd R Golub, Gad Getz, Jesse S Boehm, J Christopher Love

46) GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins (Nature Biotechnology) Sunday April 20th 2014
Leander Meuris, Francis Santens, Greg Elson, Nele Festjens, Morgane Boone, Ana, Simon Devos, Fran, Evelyn Plets, Erica Houthuys, Pauline Malinge, Giovanni Magistrelli, Laura Cons, Laurence Chatel, Bart Devreese, Nico Callewaert

47) Quantitative analysis of RNA-protein interactions on a massively parallel array reveals biophysical and evolutionary landscapes (Nature Biotechnology) Monday April 14th 2014
Jason D Buenrostro, Carlos L Araya, Lauren M Chircus, Curtis J Layton, Howard Y Chang, Michael P Snyder, William J Greenleaf

48) Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal (Nature Biotechnology) Monday April 14th 2014
Thomas S Elliott, Fiona M Townsley, Ambra Bianco, Russell J Ernst, Amit Sachdeva, Simon J Els, Lloyd Davis, Kathrin Lang, Rudolf Pisa, Sebastian Greiss, Kathryn S Lilley, Jason W Chin

49) Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption (Nature Biotechnology) Monday April 7th 2014
Benjamin L Wang, Adel Ghaderi, Hang Zhou, Jeremy Agresti, David A Weitz, Gerald R Fink, Gregory Stephanopoulos

50) Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype (Nature Biotechnology) Sunday March 30th 2014
Hao Yin, Wen Xue, Sidi Chen, Roman L Bogorad, Eric Benedetti, Markus Grompe, Victor Koteliansky, Phillip A Sharp, Tyler Jacks, Daniel G Anderson

51) Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing (Nature Biotechnology) Sunday March 23rd 2014
Adam J Shuhendler, Kanyi Pu, Lina Cui, Jack P Uetrecht, Jianghong Rao

52) The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells (Nature Biotechnology) Sunday March 23rd 2014
Cole Trapnell, Davide Cacchiarelli, Jonna Grimsby, Prapti Pokharel, Shuqiang Li, Michael Morse, Niall J Lennon, Kenneth J Livak, Tarjei S Mikkelsen, John L Rinn

53) Development and function of human innate immune cells in a humanized mouse model (Nature Biotechnology) Monday March 17th 2014
Anthony Rongvaux, Tim Willinger, Jan Martinek, Till Strowig, Sofia V Gearty, Lino L Teichmann, Yasuyuki Saito, Florentina Marches, Stephanie Halene, A Karolina Palucka, Markus G Manz, Richard A Flavell

54) Massively parallel functional annotation of 3 (Nature Biotechnology) Monday March 17th 2014
Wenxue Zhao, Joshua L Pollack, Denitza P Blagev, Noah Zaitlen, Michael T McManus, David J Erle

55) Genome-guided transcript assembly by integrative analysis of RNA sequence data (Nature Biotechnology) Monday March 17th 2014
Nathan Boley, Marcus H Stoiber, Benjamin W Booth, Kenneth H Wan, Roger A Hoskins, Peter J Bickel, Susan E Celniker, James B Brown

56) CRISPR-Cas systems for editing, regulating and targeting genomes (Nature Biotechnology) Monday March 3rd 2014
Jeffry D Sander, J Keith Joung

57) Mesenchymal stem cells: immune evasive, not immune privileged (Nature Biotechnology) Sunday February 23rd 2014
James A Ankrum, Joon Faii Ong, Jeffrey M Karp

58) Whole-genome haplotyping using long reads and statistical methods (Nature Biotechnology) Sunday February 23rd 2014
Volodymyr Kuleshov, Dan Xie, Rui Chen, Dmitry Pushkarev, Zhihai Ma, Tim Blauwkamp, Michael Kertesz, Michael Snyder

59) The binary protein-protein interaction landscape of Escherichia coli (Nature Biotechnology) Sunday February 23rd 2014
Seesandra V Rajagopala, Patricia Sikorski, Ashwani Kumar, Roberto Mosca, James Vlasblom, Roland Arnold, Jonathan Franca-Koh, Suman B Pakala, Sadhna Phanse, Arnaud Ceol, Roman H, Gabriella Siszler, Stefan Wuchty, Andrew Emili, Mohan Babu, Patrick Aloy, Rembert Pieper, Peter Uetz

60) Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice (Nature Biotechnology) Sunday February 16th 2014
Shrivats Mohan Iyer, Kate L Montgomery, Chris Towne, Soo Yeun Lee, Charu Ramakrishnan, Karl Deisseroth, Scott L Delp

61) Integrating human sequence data sets provides a resource of benchmark SNP and indel genotype calls (Nature Biotechnology) Sunday February 16th 2014
Justin M Zook, Brad Chapman, Jason Wang, David Mittelman, Oliver Hofmann, Winston Hide, Marc Salit

62) Smac mimetics and innate immune stimuli synergize to promote tumor death (Nature Biotechnology) Sunday January 26th 2014
Shawn T Beug, Vera A Tang, Eric C LaCasse, Herman H Cheung, Caroline E Beauregard, Jan Brun, Jeffrey P Nuyens, Nathalie Earl, Martine St-Jean, Janelle Holbrook, Himika Dastidar, Douglas J Mahoney, Carolina Ilkow, Fabrice Le Boeuf, John C Bell, Robert G Korneluk

63) Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface (Nature Biotechnology) Sunday January 26th 2014
Steven M Lewis, Xiufeng Wu, Anna Pustilnik, Arlene Sereno, Flora Huang, Heather L Rick, Gurkan Guntas, Andrew Leaver-Fay, Eric M Smith, Carolyn Ho, Christophe Hansen-Estruch, Aaron K Chamberlain, Stephanie M Truhlar, Elaine M Conner, Shane Atwell, Brian Kuhlman, Stephen J Demarest

64) Improving CRISPR-Cas nuclease specificity using truncated guide RNAs (Nature Biotechnology) Sunday January 26th 2014
Yanfang Fu, Jeffry D Sander, Deepak Reyon, Vincent M Cascio, J Keith Joung

65) The promise and challenge of high-throughput sequencing of the antibody repertoire (Nature Biotechnology) Sunday January 19th 2014
George Georgiou, Gregory C Ippolito, John Beausang, Christian E Busse, Hedda Wardemann, Stephen R Quake

66) Beyond model antigens: high-dimensional methods for the analysis of antigen-specific T cells (Nature Biotechnology) Sunday January 19th 2014
Evan W Newell, Mark M Davis

67) Single-molecule site-specific detection of protein phosphorylation with a nanopore (Nature Biotechnology) Sunday January 19th 2014
Christian B Rosen, David Rodriguez-Larrea, Hagan Bayley

68) Computational resources for high-dimensional immune analysis from the Human Immunology Project Consortium (Nature Biotechnology) Sunday January 19th 2014
Vladimir Brusic, Raphael Gottardo, Steven H Kleinstein, Mark M Davis

69) New dimensions in immunology (Nature Biotechnology) Sunday January 19th 2014


70) Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library (Nature Biotechnology) Tuesday December 24th 2013
Hiroko Koike-Yusa, Yilong Li, E-Pien Tan, Martin Del Castillo Velasco-Herrera, Kosuke Yusa

71) Genome-wide localization of small molecules (Nature Biotechnology) Sunday December 15th 2013
Lars Anders, Matthew G Guenther, Jun Qi, Zi Peng Fan, Jason J Marineau, Peter B Rahl, Jakob Lov, Alla A Sigova, William B Smith, Tong Ihn Lee, James E Bradner, Richard A Young

72) Reduced local mutation density in regulatory DNA of cancer genomes is linked to DNA repair (Nature Biotechnology) Sunday December 15th 2013
Paz Polak, Michael S Lawrence, Eric Haugen, Nina Stoletzki, Petar Stojanov, Robert E Thurman, Levi A Garraway, Sergei Mirkin, Gad Getz, John A Stamatoyannopoulos, Shamil R Sunyaev

73) Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids (Nature Biotechnology) Monday December 9th 2013
Bryony C Bonning, Narinder Pal, Sijun Liu, Zhaohui Wang, S Sivakumar, Philip M Dixon, Glenn F King, W Allen Miller

74) High-throughput genome scaffolding from in vivo DNA interaction frequency (Nature Biotechnology) Monday November 25th 2013
Noam Kaplan, Job Dekker

75) Systematic comparison of phenome-wide association study of electronic medical record data and genome-wide association study data (Nature Biotechnology) Monday November 25th 2013
Joshua C Denny, Lisa Bastarache, Marylyn D Ritchie, Robert J Carroll, Raquel Zink, Jonathan D Mosley, Julie R Field, Jill M Pulley, Andrea H Ramirez, Erica Bowton, Melissa A Basford, David S Carrell, Peggy L Peissig, Abel N Kho, Jennifer A Pacheco, Luke V Rasmussen, David R Crosslin, Paul K Crane, Jyotishman Pathak, Suzette J Bielinski, Sarah A Pendergrass, Hua Xu, Lucia A Hindorff, Rongling Li, Teri A Manolio, Christopher G Chute, Rex L Chisholm, Eric B Larson, Gail P Jarvik, Murray H Brilliant, Catherine A McCarty, Iftikhar J Kullo, Jonathan L Haines, Dana C Crawford, Daniel R Masys, Dan M Roden

76) Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice (Nature Biotechnology) Monday November 18th 2013
Luc Baeyens, Marie Lemper, Gunter Leuckx, Sofie De Groef, Paola Bonfanti, Geert Stang, Ruth Shemer, Christoffer Nord, David W Scheel, Fong C Pan, Ulf Ahlgren, Guoqiang Gu, Doris A Stoffers, Yuval Dor, Jorge Ferrer, Gerard Gradwohl, Christopher V E Wright, Mark Van de Casteele, Michael S German, Luc Bouwens, Harry Heimberg

77) Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells (Nature Biotechnology) Sunday November 10th 2013
Jeff Gole, Athurva Gore, Andrew Richards, Yu-Jui Chiu, Ho-Lim Fung, Diane Bushman, Hsin-I Chiang, Jerold Chun, Yu-Hwa Lo, Kun Zhang

78) Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions (Nature Biotechnology) Sunday November 3rd 2013
Joshua N Burton, Andrew Adey, Rupali P Patwardhan, Ruolan Qiu, Jacob O Kitzman, Jay Shendure

79) Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing (Nature Biotechnology) Sunday November 3rd 2013
Siddarth Selvaraj, Jesse R Dixon, Vikas Bansal, Bing Ren

80) Directed evolution of genetic parts and circuits by compartmentalized partnered replication (Nature Biotechnology) Sunday November 3rd 2013
Jared W Ellefson, Adam J Meyer, Randall A Hughes, Joe R Cannon, Jennifer S Brodbelt, Andrew D Ellington

81) Reassessing target antigens for adoptive T-cell therapy (Nature Biotechnology) Sunday October 20th 2013
Christian S Hinrichs, Nicholas P Restifo

82) Engineering dynamic pathway regulation using stress-response promoters (Nature Biotechnology) Sunday October 20th 2013
Robert H Dahl, Fuzhong Zhang, Jorge Alonso-Gutierrez, Edward Baidoo, Tanveer S Batth, Alyssa M Redding-Johanson, Christopher J Petzold, Aindrila Mukhopadhyay, Taek Soon Lee, Paul D Adams, Jay D Keasling

83) Development and validation of a clinical cancer genomic profiling test based on massively parallel DNA sequencing (Nature Biotechnology) Sunday October 20th 2013
Garrett M Frampton, Alex Fichtenholtz, Geoff A Otto, Kai Wang, Sean R Downing, Jie He, Michael Schnall-Levin, Jared White, Eric M Sanford, Peter An, James Sun, Frank Juhn, Kristina Brennan, Kiel Iwanik, Ashley Maillet, Jamie Buell, Emily White, Mandy Zhao, Sohail Balasubramanian, Selmira Terzic, Tina Richards, Vera Banning, Lazaro Garcia, Kristen Mahoney, Zac Zwirko, Amy Donahue, Himisha Beltran, Juan Miguel Mosquera, Mark A Rubin, Snjezana Dogan, Cyrus V Hedvat, Michael F Berger, Lajos Pusztai, Matthias Lechner, Chris Boshoff, Mirna Jarosz, Christine Vietz, Alex Parker, Vincent A Miller, Jeffrey S Ross, John Curran, Maureen T Cronin, Philip J Stephens, Doron Lipson, Roman Yelensky

84) A single-molecule long-read survey of the human transcriptome (Nature Biotechnology) Wednesday October 9th 2013
Donald Sharon, Hagen Tilgner, Fabian Grubert, Michael Snyder

85) Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy (Nature Biotechnology) Wednesday October 9th 2013
Yicong Wu, Peter Wawrzusin, Justin Senseney, Robert S Fischer, Ryan Christensen, Anthony Santella, Andrew G York, Peter W Winter, Clare M Waterman, Zhirong Bao, Daniel A Col, Matthew McAuliffe, Hari Shroff

86) Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins (Nature Biotechnology) Wednesday October 9th 2013
Morgan L Maeder, James F Angstman, Marcy E Richardson, Samantha J Linder, Vincent M Cascio, Shengdar Q Tsai, Quan H Ho, Jeffry D Sander, Deepak Reyon, Bradley E Bernstein, Joseph F Costello, Miles F Wilkinson, J Keith Joung

87) Strategic addition of an N-linked glycan to a monoclonal antibody improves its HIV-1 (Nature Biotechnology) Sunday October 6th 2013
Ruijiang Song, Deena A Oren, David Franco, Michael S Seaman, David D Ho

88) Identifying producers of antibacterial compounds by screening for antibiotic resistance (Nature Biotechnology) Sunday October 6th 2013
Maulik N Thaker, Wenliang Wang, Peter Spanogiannopoulos, Nicholas Waglechner, Andrew M King, Ricardo Medina, Gerard D Wright

89) Accelerating the design of biomimetic materials by integrating RNA-seq with proteomics and materials science (Nature Biotechnology) Sunday September 8th 2013
Paul A Guerette, Shawn Hoon, Yiqi Seow, Manfred Raida, Admir Masic, Fong T Wong, Vincent H B Ho, Kiat Whye Kong, Melik C Demirel, Abdon Pena-Francesch, Shahrouz Amini, Gavin Z Tay, Dawei Ding, Ali Miserez

90) Modified mRNA directs the fate of heart progenitor cells and induces vascular regeneration after myocardial infarction (Nature Biotechnology) Sunday September 8th 2013
Lior Zangi, Kathy O Lui, Alexander von Gise, Qing Ma, Wataru Ebina, Leon M Ptaszek, Daniela Sp, Huansheng Xu, Mohammadsharif Tabebordbar, Rostic Gorbatov, Brena Sena, Matthias Nahrendorf, David M Briscoe, Ronald A Li, Amy J Wagers, Derrick J Rossi, William T Pu, Kenneth R Chien

91) Locus-specific editing of histone modifications at endogenous enhancers (Nature Biotechnology) Sunday September 8th 2013
Eric M Mendenhall, Kaylyn E Williamson, Deepak Reyon, James Y Zou, Oren Ram, J Keith Joung, Bradley E Bernstein

92) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences (Nature Biotechnology) Sunday August 25th 2013
Morgan G I Langille, Jesse Zaneveld, J Gregory Caporaso, Daniel McDonald, Dan Knights, Joshua A Reyes, Jose C Clemente, Deron E Burkepile, Rebecca L Vega Thurber, Rob Knight, Robert G Beiko, Curtis Huttenhower

93) Nature-inspired design of motif-specific antibody scaffolds (Nature Biotechnology) Monday August 19th 2013
James T Koerber, Nathan D Thomsen, Brett T Hannigan, William F Degrado, James A Wells

94) Conditional targeted genome editing using somatically expressed TALENs in C. elegans (Nature Biotechnology) Monday August 19th 2013
Ze Cheng, Peishan Yi, Xiangming Wang, Yongping Chai, Guoxin Feng, Yihong Yang, Xing Liang, Zhiwen Zhu, Wei Li, Guangshuo Ou

95) MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies (Nature Biotechnology) Sunday August 11th 2013
Ryan A Langlois, Randy A Albrecht, Brian Kimble, Troy Sutton, Jillian S Shapiro, Courtney Finch, Matthew Angel, Mark A Chua, Ana Silvia Gonzalez-Reiche, Kemin Xu, Daniel Perez, Adolfo Garc, Benjamin R tenOever

96) High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity (Nature Biotechnology) Sunday August 11th 2013
Vikram Pattanayak, Steven Lin, John P Guilinger, Enbo Ma, Jennifer A Doudna, David R Liu

97) Generation of tumor-targeted human T lymphocytes from induced pluripotent stem cells for cancer therapy (Nature Biotechnology) Sunday August 11th 2013
Maria Themeli, Christopher C Kloss, Giovanni Ciriello, Victor D Fedorov, Fabiana Perna, Mithat Gonen, Michel Sadelain

98) CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering (Nature Biotechnology) Thursday August 1st 2013
Prashant Mali, John Aach, P Benjamin Stranges, Kevin M Esvelt, Mark Moosburner, Sriram Kosuri, Luhan Yang, George M Church

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